PMID: 9430523Jan 16, 1998Paper

Fasciola hepatica cysteine proteinases: immunodominant antigens in human fascioliasis

The American Journal of Tropical Medicine and Hygiene
M CórdovaJ R Espinoza

Abstract

Fasciola hepatica adult worm cysteine proteinases were active-site, affinity radio-labeled with benzyloxicarbonyl-L-tyrosine-L-alanine diazomethylketone (Z-Tyr125I-Ala-CHN2). Sera from patients with fascioliasis and from rabbits experimentally infected with F. hepatica immunoprecipitated the radiolabeled parasite cysteine proteinases in immunoelectrophoresis assays. Two purified antigens were identified as part of the complex mosaic of antigens immunoprecipitated by the sera of infected patients. These antigens (Fas1 and Fas2) have been shown to be an important part of the Fharc2 precipitin band used for serologic diagnosis in humans and cattle. They showed cysteine proteinase activity with different proteolytic specificities and partial identity in double immunodiffusion assays. The results obtained in this work show that the Fas1 and Fas2 antigens are sensitive and specific antigens for diagnosis of this serious helminthic disease in humans and other susceptible hosts.

Citations

Jul 9, 2008·The Journal of Parasitology·Tae-Yun KimJong-Yil Chai
Mar 27, 2007·Revista Do Instituto De Medicina Tropical De São Paulo·María Alejandra De AlmeidaRenzo Nino Incani
Sep 27, 2008·Journal of Helminthology·L A MarcosJ R Espinoza
Mar 21, 2013·Parasitology Research·Mahmoud Mohamed ElsibaeiBasant Osama Mohammed
Jun 22, 2017·Transactions of the Royal Society of Tropical Medicine and Hygiene·Liliya KazantsevaJose R Espinoza
Sep 24, 1999·Transactions of the Royal Society of Tropical Medicine and Hygiene·M CórdovaJ R Espinoza
Nov 19, 2002·The Journal of Parasitology·Byoung-Kuk NaTong-Soo Kim
Sep 13, 2005·Journal of Helminthology·J R EspinozaP Herrera-Velit

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