Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep

Veterinary Parasitology
A EscamillaJosé Pérez

Abstract

The aim of the present work was to evaluate the number of apoptotic eosinophils in the livers of sheep experimentally infected with Fasciola hepatica during the migratory and biliary stages of infection. Four groups (n=5) of sheep were used; groups 1-3 were orally infected with 200 metacercariae (mc) and sacrificed at 8 and 28 days post-infection (dpi), and 17 weeks post-infection (wpi), respectively. Group 4 was used as an uninfected control. Apoptosis was detected using immunohistochemistry with a polyclonal antibody against anti-active caspase-3, and transmission electron microscopy (TEM). Eosinophils were identified using the Hansel stain in serial sections for caspase-3, and by ultrastructural features using TEM. At 8 and 28 dpi, numerous caspase-3(+) eosinophils were mainly found at the periphery of acute hepatic necrotic foci. The percentage of caspase -3(+) apoptotic eosinophils in the periphery of necrotic foci was high (46.1-53.9) at 8 and 28 dpi, respectively, and decreased in granulomas found at 28 dpi (6%). Transmission electron microscopy confirmed the presence of apoptotic eosinophils in hepatic lesions at 8 and 28 dpi. At 17 wpi, apoptotic eosinophils were detected in the infiltrate surrounding some enlarged bil...Continue Reading

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Citations

Jul 25, 2017·Diseases of Aquatic Organisms·Jerko HrabarIvona Mladineo
Sep 27, 2019·International Journal of Obesity : Journal of the International Association for the Study of Obesity·Ailec Ho-PlagaroEduardo Garcia-Fuentes
Oct 7, 2017·Transboundary and Emerging Diseases·N J BeesleyD J L Williams
Sep 29, 2020·Frontiers in Immunology·Sinéad RyanSinéad Weldon
Jan 24, 2019·International Journal for Parasitology. Parasites and Wildlife·Ji-Li ZhangJi-Yu Zhang

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