FasL gene-deficient mice display a limited disruption in spermatogenesis and inhibition of mono-(2-ethylhexyl) phthalate-induced germ cell apoptosis.

Toxicological Sciences : an Official Journal of the Society of Toxicology
Yi-Chen LinJohn H Richburg

Abstract

FasL (TNFSF6, CD95L) is hypothesized to trigger testicular germ cell apoptosis that normally occurs during a distinct peripubertal period as well as in response to toxicant-induced Sertoli cell injury. To test this hypothesis, we evaluated the testis of FasL gene-deficient mice (FasL(-/-)) at two distinct developmental ages (postnatal day [PND] 28 and 44) and after toxicant-induced Sertoli cell injury. Testicular cross sections from peripubertal (PND 28) FasL(-/-) mice showed significant increases in the basal germ cell apoptotic index (AI; 20.58 +/- 4.59) as compared to the testis of C57BL/6J wild-type mice (5.16 +/- 0.08) and closely correlated with increased expression of TRAIL protein in the testis of FasL(-/-) mice. A limited, but significant, number of seminiferous tubules in the testis of PND 28 FasL(-/-) mice showed a severe loss of germ cells with only Sertoli cells present. In contrast, no apparent gross histological changes were observed in the testis of adult (PND 44) FasL(-/-) mice. However, PND 44 FasL(-/-) mice did show a 51% reduction in homogenization-resistant elongate spermatids as compared to age-matched C57BL/6J mice. Exposure of PND 28 FasL(-/-) mice to mono-(2-ethylhexyl) phthalate (MEHP), a well-describe...Continue Reading

References

Sep 1, 1978·Journal of Reproduction and Fertility·G W RobbG J Killian
Jan 1, 1984·Critical Reviews in Toxicology·J A Thomas, M J Thomas
May 31, 1996·The Journal of Biological Chemistry·R M PittiA Ashkenazi
Apr 4, 1997·Science·G PanV M Dixit
Nov 5, 1997·The Journal of Biological Chemistry·M MacFarlaneE S Alnemri
Dec 31, 1997·FEBS Letters·P SchneiderJ Tschopp
Aug 28, 1998·Science·A Ashkenazi, V M Dixit
Jul 20, 1999·The Journal of Clinical Investigation·A AshkenaziR H Schwall
May 8, 2000·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C G Print, K L Loveland
Nov 20, 2001·Molecular and Cellular Biology·A KruegerS Kirchhoff
Mar 22, 2002·Nature Reviews. Immunology·M Thome, J Tschopp
Feb 7, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Saoussen KarrayMatthieu Lévi-Strauss
Aug 20, 2004·The Biochemical Journal·Marcus E Peter
Nov 27, 2004·Biology of Reproduction·Nandini BhattacharyaKwan Hee Kim
Jan 18, 2005·Journal of Cell Science·Inna LavrikPeter H Krammer
May 6, 2006·Cell Death and Differentiation·C GarridoG Kroemer
May 9, 2006·Biochimica Et Biophysica Acta·Vladimir GogvadzeBoris Zhivotovsky
Oct 10, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Marc L HyerJohn C Reed
Dec 29, 2006·The Journal of Biological Chemistry·Pei-Li YaoJohn H Richburg
May 8, 2007·Cell·Marcus E PeterDavid H Lynch
Jun 15, 2007·Toxicologic Pathology·Susan Elmore
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Liying WangYon Rojanasakul

❮ Previous
Next ❯

Citations

Jan 10, 2016·Tissue & Cell·Mohammad Shah Alam, Masamichi Kurohmaru
Jun 7, 2014·Critical Reviews in Toxicology·Vanessa R KayWarren G Foster
Jun 7, 2012·Spermatogenesis·Jessica L Dwyer, John H Richburg
Feb 10, 2012·Spermatogenesis·Ricardo D MorenoRaúl Lagos-Cabré
Dec 3, 2014·Fertility and Sterility·Gianpiero D PalermoZev Rosenwaks
Aug 17, 2016·Environmental Health : a Global Access Science Source·Zana PercyKimberly Yolton
Aug 2, 2017·Reproduction, Fertility, and Development·Tomasz LechKatarzyna Kotarska
May 11, 2018·Reproduction : the Official Journal of the Society for the Study of Fertility·Jorine J L P VossJohn H Richburg
Jan 8, 2021·Environmental Science and Pollution Research International·Bin-Bin ZhuDe-Xiang Xu

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR

Software Mentioned

MetaMorph Imaging

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis