FasR, a novel class of transcriptional regulator, governs the activation of fatty acid biosynthesis genes in Streptomyces coelicolor

Molecular Microbiology
Ana ArabolazaHugo Gramajo

Abstract

Membrane lipid homeostasis is essential for bacterial survival and adaptation to different environments. The regulation of fatty acid biosynthesis is therefore crucial for maintaining the correct composition and biophysical properties of cell membranes. This regulation implicates a biochemical control of key enzymes and a transcriptional regulation of genes involved in lipid metabolism. In Streptomyces coelicolor we found that control of lipid homeostasis is accomplished, at least in part, through the transcriptional regulation of fatty acid biosynthetic genes. A novel transcription factor, FasR (SCO2386), controls expression of fabDHPF operon and lies immediately upstream of fabD, in a cluster of genes that is highly conserved within actinomycetes. Disruption of fasR resulted in a mutant strain, with severe growth defects and a delay in the timing of morphological and physiological differentiation. Expression of fab genes was downregulated in the fasR mutant, indicating a role for this transcription factor as an activator. Consequently, the mutant showed a significant drop in fatty acid synthase activity and triacylglyceride accumulation. FasR binds specifically to a DNA sequence containing fabDHPF promoter region, both in viv...Continue Reading

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Citations

Aug 29, 2012·Applied Microbiology and Biotechnology·Martín A HernándezHéctor M Alvarez
May 1, 2012·Applied and Environmental Microbiology·Eduardo RodriguezHugo Gramajo
Dec 26, 2012·Research in Microbiology·Alexandre GouzyStephen V Gordon
Jun 1, 2013·Molecular Microbiology·S MondinoH Gramajo
Oct 14, 2010·Molecular Microbiology·Yong-Mei Zhang, Charles O Rock
Jul 28, 2010·Molecular Microbiology·V SalzmanH Gramajo
Jan 6, 2011·FEMS Microbiology Reviews·Gabriela GagoHugo Gramajo
Feb 1, 2015·Applied Microbiology and Biotechnology·Tatjana Ilic-TomicNiko S Radulović
Dec 3, 2014·Journal of Biotechnology·Kristina IrzikLothar Eggeling
Jul 31, 2016·Applied and Environmental Microbiology·Annika RöttigAlexander Steinbüchel

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