Fast analysis of doping agents in urine by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. II: Confirmatory analysis

Journal of Chromatography. a
Flavia BadoudJ-L Veuthey

Abstract

For doping control, analyses of samples are generally achieved in two steps: a rapid screening and, in the case of a positive result, a confirmatory analysis. A two-step methodology based on ultra-high-pressure liquid chromatography coupled to a quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) was developed to screen and confirm 103 doping agents from various classes (e.g., beta-blockers, stimulants, diuretics, and narcotics). The screening method was presented in a previous article as part I (i.e., Fast analysis of doping agents in urine by ultra-high-pressure liquid chromatography-quadrupole time-of-flight mass spectrometry. Part I: screening analysis). For the confirmatory method, basic, neutral and acidic compounds were extracted by a dedicated solid-phase extraction (SPE) in a 96-well plate format and detected by MS in the tandem mode to obtain precursor and characteristic product ions. The mass accuracy and the elemental composition of precursor and product ions were used for compound identification. After validation including matrix effect determination, the method was considered reliable to confirm suspect results without ambiguity according to the positivity criteria established by the World Anti-Doping Agen...Continue Reading

References

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Sep 12, 2009·Journal of Chromatography. a·Ivano MarchiJean-Luc Veuthey

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Citations

Mar 23, 2011·Analytical and Bioanalytical Chemistry·Mario ThevisWilhelm Schänzer
Mar 7, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Irena BaranowskaJacek Baranowski
Aug 15, 2012·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Alan Hb WuKara L Lynch
Nov 14, 2015·Analytical Chemistry·Raul NicoliJean-Luc Veuthey
Oct 16, 2012·Journal of Chromatography. a·Marta Rodriguez-AllerDavy Guillarme
Aug 10, 2011·Forensic Science International : Synergy·Flavia BadoudJean-Luc Veuthey
Apr 13, 2010·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Elke Aehle, Birgit Dräger
Dec 15, 2010·Biomedical Chromatography : BMC·Lewis Couchman, Phillip E Morgan
Jan 22, 2011·Drug Testing and Analysis·Mario ThevisWilhelm Schänzer
Sep 24, 2015·Bioanalysis·Oskar GonzálezRosa M Alonso
Apr 12, 2011·Analytical and Bioanalytical Chemistry·K DeventerP Van Eenoo

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