Fast and accurate preparation fatty acid methyl esters by microwave-assisted derivatization in the yeast Saccharomyces cerevisiae

Applied Microbiology and Biotechnology
Sakda KhoomrungJens Nielsen

Abstract

We present a fast and accurate method for preparation of fatty acid methyl esters (FAMEs) using microwave-assisted derivatization of fatty acids present in yeast samples. The esterification of free/bound fatty acids to FAMEs was completed within 5 min, which is 24 times faster than with conventional heating methods. The developed method was validated in two ways: (1) through comparison with a conventional method (hot plate) and (2) through validation with the standard reference material (SRM) 3275-2 omega-3 and omega-6 fatty acids in fish oil (from the Nation Institute of Standards and Technology, USA). There were no significant differences (P>0.05) in yields of FAMEs with both validations. By performing a simple modification of closed-vessel microwave heating, it was possible to carry out the esterification in Pyrex glass tubes kept inside the closed vessel. Hereby, we are able to increase the number of sample preparations to several hundred samples per day as the time for preparation of reused vessels was eliminated. Pretreated cell disruption steps are not required, since the direct FAME preparation provides equally quantitative results. The new microwave-assisted derivatization method facilitates the preparation of FAMEs di...Continue Reading

References

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Citations

Apr 7, 2015·Biochimica Et Biophysica Acta·Raffaele NicastroPaola Coccetti
Jun 17, 2016·Molecular Biology of the Cell·Petri-Jaan LahtveeJens Nielsen
Nov 20, 2016·Biotechnology and Bioengineering·Leonie WenningVerena Siewers
Feb 22, 2017·Nature Chemical Biology·Zhiwei ZhuJens Nielsen
Jan 27, 2018·Microbial Cell Factories·Yongjun WeiJens Nielsen
Jan 24, 2018·Proceedings of the National Academy of Sciences of the United States of America·Raphael FerreiraJens Nielsen
Feb 9, 2020·Applied and Environmental Microbiology·Guoxing ZhuJianrong Wu
Nov 20, 2018·Biotechnology for Biofuels·Zhong-Peng GuoLisbeth Olsson
Dec 31, 2019·Journal of Food and Drug Analysis·Huai-Hsuan Chiu, Ching-Hua Kuo
Oct 26, 2013·Experimental Biology and Medicine·Aysha HussainAgneta Holmäng
Aug 5, 2017·Frontiers in Pharmacology·Sakda KhoomrungPravit Akarasereenont
Mar 3, 2016·NPJ Systems Biology and Applications·Eduard J KerkhovenJens Nielsen
Jan 21, 2021·International Journal of Molecular Sciences·Ionut Avramia, Sonia Amariei
Jul 9, 2014·Journal of Agricultural and Food Chemistry·Cynthia Tyburczy Srigley, Jeanne I Rader
Sep 11, 2021·Preparative Biochemistry & Biotechnology·Mohammad HomayoonfarSoroush Sardari

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