Fast and effective: intense pulse light photodynamic inactivation of bacteria.

Journal of Industrial Microbiology & Biotechnology
Tim MaischWolfgang Bäumler

Abstract

The goal of this study was to investigate the photodynamic toxicity of TMPyP (5, 10, 15, 20-Tetrakis (1-methylpyridinium-4-yl)-porphyrin tetra p-toluenesulfonate) in combination with short pulses (ms) of an intense pulse light source within 10 s against Bacillus atrophaeus, Staphylococcus aureus, Methicillin-resistant S. aureus and Escherichia coli, major pathogens in food industry and in health care, respectively. Bacteria were incubated with a photoactive dye (TMPyP) that is subsequently irradiated with visible light flashes of 100 ms to induce oxidative damage immediately by generation of reactive oxygen species like singlet oxygen. A photodynamic killing efficacy of up to 6 log(10) (>99.9999%) was achieved within a total treatment time of 10 s using a concentration range of 1-100 μmol TMPyP and multiple light flashes of 100 ms (from 20 J cm(-2) up to 80 J cm(-2)). Both incubation of bacteria with TMPyP alone or application of light flashes only did not have any negative effect on bacteria survival. Here we could demonstrate for the first time that the combination of TMPyP as the respective photosensitizer and a light flash of 100 ms of an intense pulsed light source is enough to generate sufficient amounts of reactive oxyge...Continue Reading

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Citations

Jun 7, 2015·Journal of Photochemistry and Photobiology. B, Biology·Maisch Tim
Oct 8, 2014·Scandinavian Journal of Infectious Diseases·Ewa DworniczekGrażyna Gościniak
Apr 22, 2015·Clinical Orthopaedics and Related Research·Sana S DastgheybNoreen J Hickok
May 1, 2015·Current Microbiology·Natália Norika YassunakaJane Martha Graton Mikcha
Sep 27, 2013·Virulence·Fatma VatanseverMichael R Hamblin
Aug 28, 2014·Frontiers in Microbiology·Fabian CieplikTim Maisch
Mar 20, 2013·BioMed Research International·Aleksandra TaraszkiewiczJoanna Nakonieczna
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Jan 23, 2016·Journal of Biophotonics·Anita GollmerCristina Flors
Nov 26, 2014·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Anja EichnerTim Maisch
Mar 1, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Pouriya Faraj TabriziTim Maisch
Jun 23, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Tim Maisch
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Sep 20, 2019·International Journal of Molecular Sciences·Fabienne HilgersThomas Drepper
Mar 3, 2017·Applied Microbiology and Biotechnology·Yaxin ZhangMingdong Huang
Mar 1, 2019·Comprehensive Reviews in Food Science and Food Safety·Vinayak S GhateHyun-Gyun Yuk

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