Mar 5, 2016

Fast and inexpensive detection of bacterial viability and drug effectiveness through metabolic monitoring

BioRxiv : the Preprint Server for Biology
Sondos AyyashPonnambalam Ravi Selvaganapathy

Abstract

Conventional methods for the detection of bacterial infection such as DNA or immunoassays are either expensive, time consuming, or not definitive; thus may not provide all the information sought by the medical professionals. In particular, it is difficult to obtain information about viability or drug effectiveness, which are crucial to formulate a treatment. Bacterial culture test is the gold standard because it is inexpensive and does not require extensive sample preparation, and most importantly, provides all the necessary information sought by healthcare professionals, such as bacterial presence, viability and drug effectiveness. These conventional culture methods, however, have a long turnaround time: anywhere between 1 day to 4 weeks. Here, we solve this problem by monitoring the growth of bacteria in thousands of nanowells simultaneously to identify its presence in the sample and its viability, faster. The segmentation of a sample with low bacterial concentration into thousands of nanoliter wells digitizes the samples and increases the effective concentration in those wells that contain bacteria. We monitor the metabolism of aerobic bacteria by using an oxygen sensitive fluorophore, ruthenium tris (2,2-diprydl) dichloride...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
Biologic Segmentation
Size
Tuberculosis
Pathogenic Organism
Escherichia coli K12
Tromethamine
Pharmacologic Substance
Metabolic Pathway
Monitoring - Action

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