Fast and precise quantification of serum biomarkers and simultaneous recognition of multiple diseases enabled by a stable isotope-labelled peptides assisted high-throughput MRM strategy

The Analyst
Anqi HuYi-Ou Cao

Abstract

Serum/plasma holds promise as an important source of disease-related proteins and even biomarkers in clinical practice. However, the discovery of biomarker candidates in serum/plasma remains challenging. In this study, we constructed an MS strategy that enables the fast and precise quantification of serum biomarkers through coupling a high-throughput scheduled MRM strategy with a stable isotope-labelled (SIL) peptide panel from more than 500 plasma proteins as internal standards. With this strategy, we discovered relevant serum proteins of atherosclerosis (AS), lung cancer (LC) and breast cancer (BC), which can simultaneously recognize these diseases. The results indicate that the powerful strategy we constructed has the potential for serum biomarker screening and disease detection.

References

Jul 10, 2009·Nature Biotechnology·Martin McIntosh, Matthew Fitzgibbon
Oct 19, 2013·Methods in Molecular Biology·H Alexander Ebhardt
Aug 29, 2014·Analytical Chemistry·Jun GaoShaolian Zhou
Sep 28, 2017·Molecular Systems Biology·Philipp E GeyerMatthias Mann
May 31, 2018·CA: a Cancer Journal for Clinicians·Andrew M D WolfRobert A Smith
Dec 12, 2018·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Luis M SeijoLuis M Montuenga
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal
Jul 11, 2019·Molecular & Cellular Proteomics : MCP·Ruth HüttenhainRuedi Aebersold
Sep 1, 2019·Molecular & Cellular Proteomics : MCP·Ruth HüttenhainRuedi Aebersold

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BETA
biopsy
immunodepletion

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PQ500
Skyline
Metaboanalyst

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