Fast antimicrobial susceptibility testing on Escherichia coli by metabolic heat nanocalorimetry.

Lab on a Chip
Yang LiuMartin A M Gijs

Abstract

Fast spreading of antimicrobial resistance is now considered a major global health threat. New technologies are required, enabling rapid diagnostics of bacterial infection combined with fast antimicrobial susceptibility testing (AST) for evaluating the efficiency and dosage of antimicrobial compounds in vitro. This work presents an integrated chip-based isothermal nanocalorimetry platform for direct microbial metabolic heat measurements and evaluates its potential for fast AST. Direct detection of the bacteria-generated heat allows monitoring of metabolic activity and antimicrobial action at subinhibitory concentrations in real-time. The high heat sensitivity of the platform enables bacterial growth detection within only a few hours of incubation, whereas growth inhibition upon administration of antibiotics is revealed by a decrease or the absence of the heat signal. Antimicrobial stress results in lag phase extension and metabolic energy spilling. Oxygen consumption and optical density measurements provide a more holistic insight of the metabolic state and the evolution of bacterial biomass. As a proof-of-concept, a metabolic heat-based AST study on Escherichia coli as model organism with 3 clinically relevant antibiotics is p...Continue Reading

References

Oct 1, 1987·The American Journal of Physiology·P D Walker, S V Shah
Apr 1, 1967·Journal of Bacteriology·W F Loomis, B Magasanik
Jun 23, 2001·The Journal of Antimicrobial Chemotherapy·J M Andrews
May 30, 2002·Analytical Chemistry·Erik A JohannessenJonathan M Cooper
May 3, 2003·The Journal of Clinical Investigation·Franklin D Lowy
Dec 23, 2003·Infection and Immunity·Miguel ReguéJuan M Tomás
Jun 15, 2004·International Journal of Food Microbiology·I A M SwinnenJ F Van Impe
Jun 24, 2004·Proceedings of the National Academy of Sciences of the United States of America·Francisco E TorresRichard H Bruce
Aug 19, 2007·Journal of Molecular Microbiology and Biotechnology·James B Russell
May 27, 2008·Journal of Microbiological Methods·J LerchnerT Maskow
Mar 3, 2009·Analytical Biochemistry·Michael I RechtRichard H Bruce
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Wonhee LeeMichael L Roukes
Nov 10, 2009·FEMS Microbiology Letters·Olivier BraissantAlma U Daniels
May 5, 2010·Nature Reviews. Microbiology·Michael A KohanskiJames J Collins
Sep 22, 2010·Antimicrobial Agents and Chemotherapy·Jennifer H MoffattJohn D Boyce
Jan 1, 2010·Sensors·Olivier BraissantA U Daniels
Mar 5, 2013·International Journal of Medical Microbiology : IJMM·Frida MarianaThomas Maskow
Mar 15, 2013·Journal of Clinical Microbiology·Alex van Belkum, W Michael Dunne
May 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Ana SolopovaOscar P Kuipers
Dec 3, 2014·Methods : a Companion to Methods in Enzymology·O BraissantG Bonkat
May 6, 2016·Gut Microbes·Eugene Rosenberg, Ilana Zilber-Rosenberg
Sep 28, 2016·Nature Microbiology·Rebecca SugdenSally Davies
Nov 3, 2016·The Review of Scientific Instruments·Shuyu WangLei Zuo
Dec 14, 2016·Scientific Reports·Keiran StevensonTeuta Pilizota
Dec 17, 2016·Science·Alexander HarmsKenn Gerdes
Apr 1, 2016·Journal of Pharmaceutical Analysis·Mounyr BalouiriSaad Koraichi Ibnsouda
Oct 20, 2018·Nature Reviews. Microbiology·Alex van BelkumUNKNOWN JPIAMR AMR-RDT Working Group on Antimicrobial Resistance and Rapid Diagnostic Testing

❮ Previous
Next ❯

Citations

Jan 21, 2021·International Journal of Molecular Sciences·Simone RentschlerHans-Peter Deigner
Nov 11, 2020·Analytical Chemistry·Simon F BerlandaPetra S Dittrich

❮ Previous
Next ❯

Methods Mentioned

BETA
chip
chips
optical microscopy

Software Mentioned

OriginLab®
INCfAST

Related Concepts

Related Feeds

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Related Papers

The Canadian Journal of Infectious Diseases = Journal Canadien Des Maladies Infectieuses
G G ZhanelG K Harding
Nature Reviews. Microbiology
Alex van BelkumJPIAMR AMR-RDT Working Group on Antimicrobial Resistance and Rapid Diagnostic Testing
© 2021 Meta ULC. All rights reserved