PMID: 3762331Sep 1, 1986Paper

Fast atom bombardment and tandem mass spectrometry of phosphatidylserine and phosphatidylcholine

Lipids
N J JensenM L Gross

Abstract

Fast atom bombardment (FAB) desorption of phosphatidylserine and various phosphatidylcholines produces a limited number of very informative negative ions. Especially significant is the formation of (M-H)- ions for phosphatidylserine, a compound which does not yield informative high mass ions by other ionization methods. Phosphatidylcholines do not yield (M-H)- ions but instead produce three characteristic high mass ions, (M-CH+3)-, [M-HN(CH3)+3]- and [M-HN(CH3)+3-C2H2]-. Both classes of lipids also yield anions attributed to the carboxylate components of these complex lipids. FAB desorption in combination with collisional activation allows for characterization of fragmentation and determination of structural features. Collisional activation of the carboxylate anion fragments from the complex lipids is especially informative. Structural characterization of the fatty acid chain can be achieved as the released saturated carboxylate anions undergo a highly specific 1,4-elimination of H2, which results in the losses of the elements of CH4, C2H6, C3H8 . . . in a fashion entirely consistent with the chemistry of carboxylate anions desorbed from free fatty acids. These CnH2n + 2 losses begin at the alkyl terminus and progress along the...Continue Reading

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Citations

Feb 24, 2001·Mass Spectrometry Reviews·C Cheng, M L Gross
May 10, 2001·Rapid Communications in Mass Spectrometry : RCM·P DominguesA J Ferrer-Correia
Jun 16, 2001·Rapid Communications in Mass Spectrometry : RCM·V ZabrouskovN R Knowles
May 1, 1990·Chemistry and Physics of Lipids·H BrachwitzA Hermetter
Apr 7, 1995·Journal of Chromatography. B, Biomedical Applications·S Chen, M Claeys
Sep 15, 1995·Journal of Chromatography. B, Biomedical Applications·A Kuksis, J J Myher
Dec 1, 1991·Journal of the American Society for Mass Spectrometry·M J Cole, C G Enke
Jan 1, 1991·Journal of the American Society for Mass Spectrometry·K Kayganich, R C Murphy
Mar 1, 1994·Journal of the American Society for Mass Spectrometry·K A Kayganich-Harrison, R C Murphy
Dec 1, 1995·Journal of the American Society for Mass Spectrometry·X Han, R W Gross
May 3, 2005·Journal of the American Society for Mass Spectrometry·Ying L LiFong-Fu Hsu
Sep 29, 2006·Journal of the American Society for Mass Spectrometry·Shelley N JacksonAmina S Woods
Apr 11, 2003·Journal of the American Society for Mass Spectrometry·Khalid A Al-SaadN Richard Knowles
Apr 11, 2000·Journal of the American Society for Mass Spectrometry·N Khaselev, R C Murphy
Apr 27, 2011·The Analyst·Catherine J PridmoreJohn M Sanderson
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J D MorrowL J Roberts
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Han, R W Gross
Apr 20, 2001·Biological Chemistry·C E CostelloB N Singh
Nov 19, 2010·Bioanalysis·Xinghua Guo, Ernst Lankmayr
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May 1, 1993·Biological Mass Spectrometry·C G de KosterJ Haverkamp
Feb 1, 1995·European Journal of Biochemistry·J DeutschS Rottem
Aug 13, 2011·Biochimica Et Biophysica Acta·Valerie B O'Donnell
May 17, 2011·Biochimica Et Biophysica Acta·Simon H J BrownStephen J Blanksby
Jul 28, 2009·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Zheng Cui, Michael J Thomas

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