PMID: 3766943Aug 1, 1986Paper

Fast atom bombardment combined with tandem mass spectrometry for the study of dinucleotides

Analytical Biochemistry
R L CernyL Grotjahn

Abstract

A study of mono- and dinucleotides by utilizing negative ion fast atom bombardment (FAB), metastable decomposition of (M-H)- species, and collisionally activated decomposition (CAD) of (M-H)- species is reported. Data were obtained for several complete series containing the standard nucleosides (guanosine, adenosine, cytidine, thymidine, and uridine): the 3'- and 5'-monophosphate mononucleotide series for both ribo- and 2'-deoxyribomononucleotides, all possible combinations for the 3'(-)----5'-ribodinucleotides, and all possible combinations of the 3'(-)----5',2'-deoxyribodinucleotides. The metastable and CAD spectra provide more information than the FAB mass spectra. The (M-H)- ions of all dinucleotides decompose either as metastable ions or upon collisional activation to eliminate BH (B = base) preferentially from the 3'- rather than the 5'-terminus. Isomeric dinucleotides can be distinguished on the basis of this fragmentation. To establish the identity of the base at the 5'-terminus, collisional activation is preferred. By comparing relative abundances of BH elimination observed, the inherent basicities of the nucleoside base anions can be inferred to be C- greater than A-, T-, greater than G-.

References

Aug 11, 1982·Nucleic Acids Research·L GrotjahnH Blöcker
Oct 15, 1982·Science·K L Rinehart

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Citations

Jan 1, 1996·Mass Spectrometry Reviews·E NordhoffP Roepstorff
Jan 1, 1992·Journal of the American Society for Mass Spectrometry·S A McLuckeyG L Glish
Dec 1, 1991·Genetic Analysis, Techniques and Applications·K B JacobsonS A McLuckey
Dec 31, 2003·Journal of the American Society for Mass Spectrometry·Rambod Daneshfar, John S Klassen
Mar 27, 2007·Journal of the American Society for Mass Spectrometry·Selina T M Monn, Stefan Schürch
Apr 29, 2008·Journal of the American Society for Mass Spectrometry·Janna AnichinaDiethard K Bohme
Sep 19, 2008·Journal of the American Society for Mass Spectrometry·Teng-Yi HuangScott A McLuckey
Feb 10, 2009·Journal of the American Society for Mass Spectrometry·Adrien NyakasStefan Schürch
Nov 10, 2009·Journal of the American Society for Mass Spectrometry·Dorothée BalbeurEdwin De Pauw
Feb 24, 2001·Journal of the American Society for Mass Spectrometry·K X WanM L Gross
Feb 1, 1995·Journal of the American Society for Mass Spectrometry·S Habibi-Goudarzi, S A McLuckey
Jan 8, 1999·Journal of the American Society for Mass Spectrometry·L A MarzilliP Vouros
Feb 28, 2013·Chemical Reviews·Natalia TretyakovaSrikanth Kotapati
Nov 15, 2012·Mass Spectrometry Reviews·Catherine Silvestri, Jennifer S Brodbelt
May 15, 1988·Biomedical & Environmental Mass Spectrometry·H Moser, G W Wood
Aug 1, 1987·Rapid Communications in Mass Spectrometry : RCM·J J DinoD G Kaufman
Apr 2, 2010·Physical Chemistry Chemical Physics : PCCP·G AravindL H Andersen
Dec 1, 2005·Journal of Mass Spectrometry : JMS·Corinne BuréAgnès F Delmas
Nov 25, 2014·Angewandte Chemie·Annalisa ArcellaModesto Orozco
Jun 15, 1996·Analytical Chemistry·A L BurlingameS J Gaskell

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