Fast capture and multiplexed detection of short multi-arm DNA stars in solid-state nanopores

Nanoscale
Liqun HeVincent Tabard-Cossa

Abstract

Fast and multiplexed detection of low-abundance disease biomarkers at the point-of-need would transform medicine. Nanopores have gained attention as single-molecule counters to electrically detect a range of biological molecules in a handheld format, but challenges remain before diagnostic applications can emerge. For solid-state nanopore sensors, the specificity of the ionic current signatures and the rate of target capture required to simultaneously recognize and rapidly count a mixture of molecular targets in a complex sample are active areas of research. Herein, we study the capture and translocation characteristics of short N-arm star shaped DNA nanostructures to evaluate their potential as a family of surrogate label molecules for biomarkers of interest, designed for fast and reliable multiplexed detection based on conductance blockages. Simple hybridization of a varying number of short, easily synthesized 50 bp ssDNA strands allows the number of arms in the star shape DNA to be controlled from N = 3 to 12. By introducing more arms to the nanostructures, we show that we can controllably increase the nanopore signal-to-noise ratio for a range of pore sizes, producing conductance blockages which increase linearly with the n...Continue Reading

References

Jun 15, 2007·Journal of the American Chemical Society·Xing Wang, Nadrian C Seeman
Dec 22, 2009·Nature Nanotechnology·Meni WanunuAmit Meller
Jul 7, 2011·Nanotechnology·Stefan W KowalczykCees Dekker
Jan 11, 2012·Nano Letters·Stefan W KowalczykCees Dekker
Feb 23, 2012·Nano Letters·Alon SingerAmit Meller
Dec 12, 2012·Analytical Chemistry·David R Walt
Jan 9, 2014·ACS Nano·Arvind BalijepalliCanute Vaz
Mar 25, 2014·PloS One·Harold KwokVincent Tabard-Cossa
Nov 25, 2014·Biophysical Journal·Spencer CarsonMeni Wanunu
Dec 6, 2014·Clinical Chemistry·Hagan Bayley
Nov 13, 2015·PloS One·Allison H SquiresAmit Meller
Feb 4, 2016·Nature·Joshua QuickMiles W Carroll
Apr 5, 2016·Nature Nanotechnology·Nicholas A W Bell, Ulrich F Keyser
May 7, 2016·PloS One·Trevor J MorinWilliam B Dunbar
Nov 1, 2016·Analytical Chemistry·Jacob H ForstaterArvind Balijepalli
Dec 15, 2016·Chemical Communications : Chem Comm·Jinglin KongUlrich F Keyser
Aug 26, 2017·ACS Nano·Mohammad Amin AlibakhshiMeni Wanunu
Oct 11, 2017·Chemical Communications : Chem Comm·Yao LinYi-Tao Long
Nov 1, 2017·Nano Letters·Kyle BriggsVincent Tabard-Cossa
Nov 29, 2017·ACS Sensors·Eric BeamishMichel Godin
Jul 11, 2018·Lab on a Chip·Bonhye KooHarold G Monbouquette

❮ Previous
Next ❯

Citations

Apr 17, 2020·The Journal of Chemical Physics·Le Qiao, Gary W Slater
Aug 25, 2020·Nanoscale·Eric BeamishMichel Godin
May 11, 2021·ACS Applied Materials & Interfaces·Yi-Yan BaiZhi-Ling Zhang
Sep 11, 2021·Nature Communications·Liqun HeVincent Tabard-Cossa

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
chip
chips

Software Mentioned

MOSAIC
LabView

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.