Fast CE analysis of adrenergic amines in different parts of Citrus aurantium fruit and dietary supplements

Journal of Separation Science
Laura MercoliniMaria Augusta Raggi

Abstract

A CE method has been developed for the simultaneous analysis of the adrenergic amines synephrine, octopamine and tyramine in Citrus aurantium (bitter orange) fruit extracts and in dietary supplements. The analytes were separated on a fused silica capillary (50 microm id, 40.0 cm effective length, 48.5 cm total length) using a BGE composed of phosphate buffer (pH 2.5, 50 mM) and applying a 30 kV potential. The samples were injected hydrodynamically at 50 mbar for 25 s. The use of photodiode array detection (lambda=195 nm) allowed the quantification of the analytes and the control of peak purity. The method has been fully validated, obtaining satisfactory values of precision and extraction yield. The analytes are extracted with water from the dried whole fruits or fruit parts (endocarp, mesocarp and exocarp) or from the commercial formulations and directly injected into the CE apparatus. The results obtained were satisfactory in terms of precision (RSD < 5.7%) and accuracy (recovery > 89%). Thus, the method has demonstrated to be suitable for the qualitative and quantitative determination of synephrine, octopamine and tyramine in C. aurantium extracts, for dietary supplement quality control and for food adulteration identification.

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Citations

Sep 10, 2011·Oxidative Medicine and Cellular Longevity·Sidney J StohsMohd Shara
Mar 7, 2014·Biological & Pharmaceutical Bulletin·Xuxu ZhengXiaohong Deng
Jul 3, 2013·Biomedical Chromatography : BMC·Szymon DziombaTomasz Bączek
Apr 12, 2011·Phytotherapy Research : PTR·Sidney J StohsMohd Shara
Mar 29, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Sidney J Stohs
Dec 9, 2014·Journal of Pharmaceutical and Biomedical Analysis·Michele ProttiLaura Mercolini
Feb 6, 2017·Journal of Exercise Nutrition & Biochemistry·Jong-Hee Kim, Yoonjung Park
Jan 10, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Carmen MannucciGioacchino Calapai

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