Fast closure of N-terminal long loops but slow formation of β strands precedes the folding transition state of Escherichia coli adenylate kinase

Biochemistry
Tomer OreviElisha Haas

Abstract

The nature of the earliest steps of the initiation of the folding pathway of globular proteins is still controversial. To elucidate the role of early closure of long loop structures in the folding transition, we studied the folding kinetics of subdomain structures in Escherichia coli adenylate kinase (AK) using Förster type resonance excitation energy transfer (FRET)-based methods. The overall folding rate of the AK molecule and of several segments that form native β strands is 0.5 ± 0.3 s(-1), in sharp contrast to the 1000-fold faster closure of three long loop structures in the CORE domain. A FRET-based "double kinetics" analysis revealed complex transient changes in the initially closed N-terminal loop structure that then opens and closes again at the end of the folding pathway. The study of subdomain folding in situ suggests a hierarchic ordered folding mechanism, in which early and rapid cross-linking by hydrophobic loop closure provides structural stabilization at the initiation of the folding pathway.

References

Jan 1, 1992·Annual Review of Biophysics and Biomolecular Structure·S W Englander, L Mayne
Nov 14, 1986·Science·J F Leszczynski, G D Rose
Jun 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S C Harrison, R Durbin
Jan 23, 1973·Biochemical and Biophysical Research Communications·S S Ristow, D B Wetlaufer
Aug 1, 1995·Proteins·S Govindarajan, R A Goldstein
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K A DillH S Chan
Mar 31, 1999·Trends in Biochemical Sciences·R L Baldwin, G D Rose
Apr 14, 1999·Nature Structural Biology·R M Ionescu, C R Matthews
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·W A EatonJ Hofrichter
Jan 9, 2003·Trends in Biochemical Sciences·Valerie Daggett, Alan R Fersht
May 28, 2003·Journal of Molecular Biology·Thomas R Weikl, Ken A Dill
Apr 21, 2004·Nature Structural & Molecular Biology·Kresten Lindorff-LarsenChristopher M Dobson
Jul 1, 2005·Protein Science : a Publication of the Protein Society·Daisuke Kihara
Sep 2, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ying WuOsman Bilsel
Apr 23, 2013·Proceedings of the National Academy of Sciences of the United States of America·Wenbing HuS Walter Englander

❮ Previous
Next ❯

Citations

Sep 27, 2014·Proceedings of the National Academy of Sciences of the United States of America·Laura E RosenSusan Marqusee
Dec 22, 2015·Current Opinion in Structural Biology·Satoshi TakahashiHiroyuki Oikawa
Aug 11, 2015·Biochemistry·Rama Reddy Goluguri, Jayant B Udgaonkar
Nov 4, 2016·The Journal of Physical Chemistry. B·Fernando Bergasa-Caceres, Herschel A Rabitz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Hereditary Sensory Autonomic Neuropathy

Hereditary Sensory Autonomic Neuropathies are a group of inherited neurodegenerative disorders characterized clinically by loss of sensation and autonomic dysfunction. Here is the latest research on these neuropathies.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Landau-Kleffner Syndrome

Landau Kleffner syndrome (LKS), also called infantile acquired aphasia, acquired epileptic aphasia, or aphasia with convulsive disorder, is a rare childhood neurological syndrome characterized by the sudden or gradual development of aphasia (the inability to understand or express language) and an abnormal electroencephalogram. Discover the latest research on LKS here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Regulation of Vocal-Motor Plasticity

Dopaminergic projections to the basal ganglia and nucleus accumbens shape the learning and plasticity of motivated behaviors across species including the regulation of vocal-motor plasticity and performance in songbirds. Discover the latest research on the regulation of vocal-motor plasticity here.