Fast determination of arsenic species and total arsenic in urine by HPLC-ICP-MS: concentration ranges for unexposed german inhabitants and clinical case studies

Journal of Analytical Toxicology
Peter Heitland, Helmut D Köster

Abstract

A fast and reliable high-pressure liquid chromatography (HPLC)-inductively coupled plasma-mass spectrometry (ICP-MS) routine method was developed for the determination of inorganic arsenic [As(III) and As(V)], organic monomethylarsonate [MMA(V)], dimethylarsinate [DMA(V)], and arsenobetaine (As-B) in human urine. The complete method validation is described, including internal and external quality assurance. Limits of quantification for the As species are 0.1 microg/L, which is sufficient to determine background concentrations of the arsenic species in human urine. Additionally, total As in all urine samples was determined by conventional ICP-MS. Mean concentrations for 82 non-exposed inhabitants from northern Germany are 12.7, 5.9, 4.0, 0.23, 0.52, and 0.17 microg/L for total As, As-B, DMA(V), As(III), MMA(V), and As(V), respectively. Approximately 15% of the total As was not identified by the anion exchange HPLC-ICP-MS method, and could be other As metabolites in urine. Two case studies underline the need for As speciation, especially when total urinary arsenic concentrations are elevated. In the first case, we investigated the effect of seafood consumption on the concentration of different arsenic species in urine for differe...Continue Reading

Citations

Sep 24, 2010·Analytical and Bioanalytical Chemistry·Jackie Morton, Elizabeth Leese
May 26, 2009·Archives of Environmental Contamination and Toxicology·Byung-Sun ChoiJung-Duck Park
Oct 25, 2008·International Journal of Hygiene and Environmental Health·Peter Heitland, Helmut D Köster
May 28, 2013·Critical Reviews in Toxicology·Ernst M BomhardSamuel M Cohen
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