Fast determination of ethambutol in pharmaceutical formulations using capillary electrophoresis with capacitively coupled contactless conductivity detection

Electrophoresis
José Alberto Fracassi da SilvaMarcone Augusto Leal de Oliveira

Abstract

A method for the determination of ethambutol (EMB), a first-line drug against tuberculosis, based on CE with capacitively coupled contactless conductivity detection is proposed. The separation of EMB and its main product of degradation were achieved in less than 3 min with a resolution of 2.0 using a BGE composed of 50 mmol/L histidine and 30 mmol/L MES, pH 6.30. By raising the pH to 8.03, the analysis time was reduced to 1.0 min, but with a significant loss of resolution (0.7). Using the best separation conditions, linearity of 0.9976 (R(2), five data points), sensitivity of 1.26x10(-4) V min mumol(-1) L, and LOD and quantification of 23.5 and 78.3 mumol/L, respectively, were obtained. Recoveries at four levels of concentration ranged from 95 to 102% and the concentration range studied ranged from 100 to 500 mumol/L. The results obtained for the determination of EMB in pharmaceutical formulations were compared with those obtained by using CE with photometric detection.

References

Jul 8, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·P ChenevierR Farinotti
Oct 3, 2002·Journal of Clinical Densitometry : the Official Journal of the International Society for Clinical Densitometry·Mona R ArekatArnold M Moses
Apr 4, 2006·Current Opinion in Pulmonary Medicine·Marcus Vinícius Nora de Souza
Dec 25, 2007·Analytica Chimica Acta·Pavel Kubán, Peter C Hauser
Jan 29, 2008·Recent Patents on Anti-infective Drug Discovery·Marcus Vinícius Nora de Souza
Feb 1, 1998·Analytical Chemistry·A J ZemannG K Bonn

❮ Previous
Next ❯

Citations

Dec 14, 2011·Analytical Chemistry·Matthew GeigerMichael T Bowser
Jul 25, 2012·Journal of Chromatography. a·Jonas J P MarkFrank-Michael Matysik
May 4, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Wen-Ying WuChang-Feng Li
Sep 4, 2015·Journal of Separation Science·Camila Dalben Madeira CamposJosé Alberto Fracassi da Silva

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antitubercular Agents (ASM)

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.

Antitubercular Agents

Antitubercular agents are pharmacologic agents for treatment of tuberculosis. Discover the latest research on antitubercular agents here.