Fast determination of hydroxylated polychlorinated biphenyls in human plasma by online solid phase extraction coupled to liquid chromatography-tandem mass spectrometry

Analytica Chimica Acta
Natalia QuineteThomas Schettgen

Abstract

Hydroxylated polychlorinated biphenyls (OH-PCBs) have been shown to be strongly retained in human blood causing endocrine-related toxicity, particularly on the thyroid system. Traditionally, analytical methods for the determination of OH-PCBs require labor-intensive and long-time consuming sample preparation with several extraction, evaporation and cleanup procedures steps and, in some cases, derivatization prior to the analysis by gas or liquid chromatography-mass spectrometry (GC-MS or LC-MS). The present study developed and validated a novel, sensitive and high throughput online solid phase extraction (SPE) method coupled to LC-tandem mass spectrometry (MS/MS) for the separation and quantitation of relevant congeners of OH-PCBs in human plasma. The developed method presented limits of quantification (LOQ) ranging from 0.02 to 0.5 ng mL(-1) and extraction recoveries from 71 to 134% for all congeners, requiring small amount of sample (only 100 μL) and minimal sample preparation. In order to evaluate the applicability of the method, preliminary tests (N = 93) were conducted in plasma from individuals occupationally exposed to very high levels of PCBs in a German cohort. Penta-through hepta-chlorinated OH-PCBs were the predomina...Continue Reading

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Jun 9, 2014·Analytical and Bioanalytical Chemistry·Natalia QuineteThomas Kraus

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Citations

May 22, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Shintaro MiseHideyuki Inui
Jul 12, 2016·International Journal of Hygiene and Environmental Health·André EsserThomas Kraus
Oct 27, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Patrice DufourCorinne Charlier
Mar 20, 2019·International Journal of Environmental Research and Public Health·Petra Maria GaumJessica Lang
Jul 31, 2019·Environmental Science and Pollution Research International·Panithi SaktrakulklaKeri C Hornbuckle
Jul 8, 2020·International Journal of Environmental Research and Public Health·Petra Maria GaumJessica Lang
Jun 20, 2018·International Journal of Mass Spectrometry·Kendra J AdamsFrancisco Fernandez-Lima
May 3, 2021·The Science of the Total Environment·Yanlin LiJerald L Schnoor

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