Fast Fourier transform-based analysis of second-harmonic generation image in keratoconic cornea

Investigative Ophthalmology & Visual Science
Wen LoHsin-Yuan Tan

Abstract

To qualify and quantify structural alterations in keratoconic corneas ex vivo by the use of second-harmonic generation (SHG) microscopy and two-dimensional fast Fourier transform (2D-FFT) analysis. Two keratoconic and three normal human corneal specimens were imaged with a multiphoton microscope. Forward and backward second-harmonic generation images (FSHG and BSHG) of corneal stroma were obtained at high resolution and at different depths. The SHG images were further analyzed with a 2D-FFT algorithm to quantify the texture and orientation changes of collagen fibers. The results showed that the abnormality of collagen architecture was found through the whole layer of stroma. 2D-FFT analysis of SHG cornea images provided fiber orientation direction and an aspect ratio (AR) as a quantitative measure of fiber direction determination. It was found that for keratoconic cornea the average AR values are statistically greater than those of the normal cornea: 1.66 ± 0.42 (case I keratoconic cornea), 1.72 ± 0.44 (case II keratoconic cornea), and 1.34 ± 0.16 (average of three normal human corneas). Furthermore, the fiber directions determined by 2D-FFT analysis of BSHG and FSHG images were strongly correlated for large AR values (AR > 2)....Continue Reading

Citations

Mar 31, 2015·Experimental Eye Research·Andrew J QuantockJames V Jester
Apr 12, 2017·Scientific Reports·Danielle M RobertsonMeifang Zhu
Aug 28, 2019·Biomedical Optics Express·M AlizadehP Loza-Alvarez
Aug 30, 2019·Journal of Biophotonics·Sheng-Lin LeeChen-Yuan Dong
Jul 12, 2019·Translational Vision Science & Technology·Francisco J ÁvilaJuan M Bueno
Jul 14, 2019·Scientific Reports·Francisco J ÁvilaJuan M Bueno
Aug 27, 2015·Biomedical Optics Express·Marco LombardoGiuseppe Lombardo
Feb 14, 2019·BioMed Research International·Juan M BuenoM Carmen Martínez-García
Jun 26, 2021·Biomedical Optics Express·Chung-Hwan ChenChi-Hsiang Lien
Nov 13, 2017·ACS Biomaterials Science & Engineering·Sheng-Lin LeeChen-Yuan Dong

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