Fast gas chromatography-negative-ion chemical ionization mass spectrometry with microscale volume sample preparation for the determination of benzodiazepines and alpha-hydroxy metabolites, zaleplon and zopiclone in whole blood

Journal of Mass Spectrometry : JMS
Teemu GunnarP Lillsunde

Abstract

Fast gas chromatography/negative-ion chemical ionization mass spectrometric (GC/NICI-MS) assay combined with rapid and nonlaborious sample preparation is presented for the simultaneous determination of benzodiazepines and alpha-hydroxy metabolites, zaleplon and zopiclone in whole blood. The compounds were extracted from 100 microl of whole blood by simultaneous multitube, microscale liquid-liquid extraction (LLE) and derivatized by N-methyl-N-(tert-butyldimethylsilyl)trifluoroacetamide (MTBSTFA), without the need for the time-consuming concentration stage. In the analytical separation, various parameters of fast GC/NICI-MS were applied, e.g. the use of hydrogen as a GC carrier gas, a high carrier gas velocity, a small film thickness of the analytical column, fast MS data acquisition, fast temperature ramping, and high initial and final temperatures of GC column. Sensitive identification, screening and quantitation of 18 compounds of interest were achieved in chromatographic separation in only 4.40 min. Accurate and reproducible results were obtained by using five different and carefully selected deuterated analogues on the basis of the chemical properties of the target analytes. Nevertheless, for alpha-OH-midazolam, and for bro...Continue Reading

References

May 1, 1992·Journal of Pharmaceutical and Biomedical Analysis·H GjerdeA S Christophersen
Aug 1, 1997·Clinical Pharmacokinetics·A G Fraser
Dec 9, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·V CirimeleB Ludes
Aug 13, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·O H Drummer
Oct 21, 1998·Therapeutic Drug Monitoring·A D Fraser
Jun 24, 2000·Journal of Chromatography. a·M M van DeurseC A Cramers
Jun 13, 2002·Journal of Chromatography. a·Stephane PirnayStéphane Bouchonnet
May 3, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Christian GiroudPatrice Mangin
May 16, 2003·European Journal of Mass Spectrometry·John M Halket, Vladimir G Zaikin
Jan 6, 2005·The International Journal of Neuropsychopharmacology·Christian HaasenJens Reimer
Apr 28, 2006·Current Opinion in Psychiatry·Heather Ashton

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Citations

Sep 2, 2008·Analytical and Bioanalytical Chemistry·Hans H Maurer
Mar 6, 2010·Analytical and Bioanalytical Chemistry·P FernándezP Cabarcos
Jun 21, 2007·Journal of Analytical Toxicology·Michael L SmithMarilyn A Huestis
Feb 7, 2012·Bioanalysis·Milena Araújo Tonon, Pierina Sueli Bonato
Oct 26, 2011·Forensic Science International : Synergy·Tom BlencowePirjo Lillsunde
May 3, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ruut KaartamaMarko Lehtonen
May 4, 2010·Forensic Science International : Synergy·Gunnel H NilssonJohan Ahlner
Dec 31, 2008·Accident; Analysis and Prevention·Karoliina K OjaniemiAini I Ostamo
Jan 8, 2008·Journal of Chromatography. a·Erwin Kaal, Hans-Gerd Janssen
Apr 25, 2013·Drug Testing and Analysis·Erkki VuoriIlkka Ojanperä
Nov 10, 2010·Biomedical Chromatography : BMC·Stacy D Brown, Tyler C Melton
Jul 25, 2013·Drug Testing and Analysis·Terhi Launiainen, Ilkka Ojanperä
Mar 18, 2015·Journal of Pharmaceutical and Biomedical Analysis·Karolina PersonaWojciech Piekoszewski
Mar 7, 2012·Forensic Science International : Synergy·Pirkko KriikkuIlkka Ojanperä
Jun 11, 2011·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Juseop KangMin-Young Jun
May 30, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Eshwar Jagerdeo, Jason E Schaff
May 28, 2019·Drug Testing and Analysis·Raimo A Ketola, Ilkka Ojanperä
Feb 13, 2020·Mass Spectrometry Reviews·Scott A BordenPierangela Palma
Mar 28, 2008·Therapeutic Drug Monitoring·Pirjo Lillsunde
May 18, 2007·Analytical Chemistry·T A BrettellJ R Almirall

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