Fast Hadamard transform capillary electrophoresis for on-line, time-resolved chemical monitoring

Analytical Chemistry
Kevin L BraunCraig A Aspinwall

Abstract

We report a new approach for collecting and deconvoluting the data in Hadamard transform capillary electrophoresis, referred to as fast Hadamard transform capillary electrophoresis (fHTCE). Using fHTCE, total analysis times can be reduced by up to 48% per multiplexed separation compared to conventional Hadamard transform capillary electrophoresis (cHTCE) while providing comparable signal-to-noise ratio enhancements. In fHTCE, the sample is injected following a pseudorandom pulsing sequence derived from the first row of a simplex matrix (S-matrix) in contrast to cHTCE, which utilizes a sequence of twice the length. In addition to the temporal savings provided by fHTCE, a 50% reduction in sample consumption is also realized due to the decreased number of sample injections. We have applied fHTCE to the analysis of mixtures of neurotransmitters and related compounds to yield improved signal-to-noise ratios with a total analysis time under 10 s. In addition, we demonstrate the capability of fHTCE to perform time-resolved monitoring of changes in the concentration of model neurochemical compounds.

References

Jul 4, 1997·Journal of Chromatography. B, Biomedical Sciences and Applications·S TucciL Hernandez
Jun 1, 2002·Analytical Chemistry·Takashi KanetaTotaro Imasaka
Apr 23, 2003·Analytical Chemistry·Kazuki HataTotaro Imasaka
Dec 31, 2003·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Takashi KanetaTotaro Imasaka
May 29, 2004·Analytical Chemistry·Jennifer A McReynolds, Scott A Shippy
Jul 31, 2004·Analytical Chemistry·Kazuki HataTotaro Imasaka
Dec 1, 1999·Analytical Chemistry·T KanetaT Imasaka
Jun 1, 1999·Analytical Chemistry·H J CrabtreeA Manz

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Citations

Feb 6, 2008·The Review of Scientific Instruments·Jeffrey W Hudgens, Denis E Bergeron
May 7, 2014·Journal of the American Society for Mass Spectrometry·Spencer A ProstSamuel H Payne
May 2, 2016·Journal of Chromatography. a·Alexander F Siegle, Oliver Trapp
Sep 1, 2016·Journal of Chromatography. a·Kerstin ZawatzkyChristopher J Welch
Oct 13, 2015·Journal of Separation Science·Alexander F Siegle, Oliver Trapp
Aug 7, 2018·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Takashi Kaneta
Jun 23, 2007·Angewandte Chemie·Oliver Trapp
Jun 12, 2018·Analytical Chemistry·Sebastian PallmannOliver Trapp

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