Fast hemicellulose quantification via a simple one-step acid hydrolysis

Biotechnology and Bioengineering
Xiadi GaoCharles E Wyman

Abstract

As the second most common polysaccharides in nature, hemicellulose has received much attention in recent years for its importance in biomass conversion in terms of producing high yields of fermentable sugars and value-added products, as well as its role in reducing biomass recalcitrance. Therefore, a time and labor efficient method that specifically analyzes hemicellulose content would be valuable to facilitate the screening of biomass feedstocks. In this study, a one-step acid hydrolysis method was developed, which applied 4 wt% sulfuric acid at 121°C for 1 h to rapidly quantify XGM (xylan + galactan + mannan) contents in various types of lignocellulosic biomass and model hemicelluloses. This method gave statistically identical results in XGM contents compared to results from conventional two-step acid hydrolysis while significantly shortening analysis time.

References

Oct 9, 1999·Biotechnology Progress·L R LyndT U Gerngross
Sep 5, 2002·Microbiology and Molecular Biology Reviews : MMBR·Lee R LyndIsak S Pretorius
Apr 17, 2003·Journal of Industrial Microbiology & Biotechnology·Badal C Saha
Aug 23, 2005·Bioresource Technology·Charles E WymanY Y Lee
Jan 28, 2006·Science·Arthur J RagauskasTimothy Tschaplinski
Jan 28, 2006·Science·Alexander E FarrellDaniel M Kammen
Feb 10, 2007·Science·Michael E HimmelThomas D Foust
Feb 13, 2007·Applied Microbiology and Biotechnology·Bärbel Hahn-HägerdalMarie F Gorwa-Grauslund
Feb 27, 2007·Trends in Biotechnology·Charles E Wyman
Apr 1, 2008·Current Opinion in Plant Biology·William S York, Malcolm A O'Neill
Mar 17, 2010·Journal of Visualized Experiments : JoVE·Cliff E FosterMarkus Pauly
Jul 31, 2010·Journal of Agricultural and Food Chemistry·Justin B SluiterDavid W Templeton
Sep 11, 2010·Biotechnology and Bioengineering·Jaclyn D DeMartiniCharles E Wyman
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Michael H StuderCharles E Wyman
Jan 1, 2011·Annual Review of Chemical and Biomolecular Engineering·Shishir P S ChundawatBruce E Dale
Jul 31, 2012·Methods in Molecular Biology·Stephen R DeckerAngela Ziebell
Aug 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Breeanna R UrbanowiczWilliam S York

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Citations

Jun 10, 2014·Biotechnology and Bioengineering·Stefan Bauer, Ana B Ibáñez
Aug 23, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ademar MorettiAna P Alonso
Apr 4, 2018·Environmental Science and Pollution Research International·Medhavi GuptaGeorge Nakhla
Jul 5, 2017·Bioprocess and Biosystems Engineering·Manisha A KhedkarSandip B Bankar
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ji-Woo HongJin-Woo Kim
Aug 15, 2021·International Journal of Biological Macromolecules·Ting WangQiang Yong

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