Fast High-Resolution Laser Ablation-Inductively Coupled Plasma Mass Spectrometry Imaging of the Distribution of Platinum-Based Anticancer Compounds in Multicellular Tumor Spheroids

Analytical Chemistry
Sarah TheinerGunda Koellensperger

Abstract

Multicellular tumor spheroid models serve as an important three-dimensional in vitro cell model system as they mimic the complex tumor microenvironment and thus have contributed to valuable assays in drug discovery studies. In this study, we present a state-of-the-art laser ablation-inductively coupled plasma mass spectrometry (LA-ICPMS) setup for high spatial resolution elemental imaging of multicellular tumor spheroids and an approach to account for variations in cell density. A low dispersion LA-ICPMS setup was employed, providing accelerated throughput and high sensitivity and permitting a lateral image resolution down to ∼2.5 μm for phosphorus and platinum in HCT116 colon cancer spheroids upon treatment with the clinically used anticancer drug oxaliplatin. Phosphorus was introduced as scalar to compensate for differences in cell density and tissue thickness and the Pt/P ratios together with the high resolution adopted in our approach allows the differentiation of platinum accumulation within each part of the morphology of the tumor spheroids (layers of proliferating, quiescent, and necrotic cells).

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Citations

Mar 8, 2018·Metallomics : Integrated Biometal Science·Matthias H M KloseBernhard K Keppler
Dec 1, 2018·Metallomics : Integrated Biometal Science·Theodora J Stewart
Mar 1, 2019·Medical Mycology·Yanan ZhaoDavid S Perlin
May 17, 2019·Analytical and Bioanalytical Chemistry·Marta Costas-RodríguezFrank Vanhaecke
Jun 22, 2019·Rapid Communications in Mass Spectrometry : RCM·Naoyuki Nakada, Yasuo Kuroki
Oct 2, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Jan MichálekAdam Pruška
Jun 12, 2020·Metallomics : Integrated Biometal Science·Sarah TheinerGunda Koellensperger
Nov 18, 2018·Analytical and Bioanalytical Chemistry·Katharina HalbachThorsten Reemtsma
Nov 3, 2020·Dalton Transactions : an International Journal of Inorganic Chemistry·Sophia HarringerBernhard K Keppler
Feb 2, 2021·Analytical and Bioanalytical Chemistry·Katharina HalbachStephan Wagner
Feb 4, 2021·Current Opinion in Chemical Biology·Sarah TheinerGunda Koellensperger
May 7, 2021·Analytical and Bioanalytical Chemistry·Andreas SchweikertGunda Koellensperger
May 22, 2021·Chemical Reviews·Philip A DobleDavid Clases
Nov 21, 2018·Journal of the American Society for Mass Spectrometry·Antonín BednaříkJan Preisler
Jan 15, 2022·Analytical Chemistry·Yu WangYan Zhou

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