Fast identification of dermatophytes by MALDI-TOF/MS using direct transfer of fungal cells on ground steel target plates

Mycoses
Keith C da CunhaLionel Fontao

Abstract

Dermatophytes cause human infections limited to keratinised tissues. We showed that the direct transfer method allows reliable identification of non-dermatophytes mould and yeast by MALDI-TOF/MS. We aimed at assessing whether the direct transfer method can be used for dermatophytes and whether an own mass spectra library would be superior to the Bruker library. We used the Bruker Biotyper to build a dermatophyte mass spectra library and assessed its performance by 1/testing a panel of mass spectrum produced with strains genotypically identified and, 2/comparing MALDI-TOF/MS identification to morphology-based methods. Identification of dermatophytes using the Bruker library is poor. Our library provided 97% concordance between ITS sequencing and MALDI-TOF/MS analysis with a panel of 1104 spectra corresponding to 276 strains. Direct transfer method using unpolished target plates allowed proper identification of 85% of dermatophytes clinical isolates most of which were common dermatophytes. A homemade dermatophyte MSP library is a prerequisite for accurate identification of species absent in the Bruker library but it also improves identification of species already listed in the database. The direct deposit method can be used to id...Continue Reading

References

Dec 1, 1996·The British Journal of Dermatology·F Baudraz-RosseletE Frenk
Mar 7, 2007·Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG·Pietro NenoffYvonne Gräser
Nov 6, 2007·Experimental Dermatology·Marcel ErhardJohannes Wöstemeyer
Apr 26, 2008·Journal of Medical Microbiology·Hsin Chieh LiTsung Chain Chang
Aug 17, 2010·International Journal of Medical Microbiology : IJMM·Françoise SymoensMichel Monod
Jul 6, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M GautierR Piarroux
Sep 13, 2014·International Journal of Molecular Sciences·Adriana CalderaroFlora De Conto
Apr 29, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Arnaud RiatJacques Schrenzel
Apr 12, 2016·Mycoses·Carole CassagneRenaud Piarroux
Jun 4, 2016·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C KupschY Gräser
Oct 27, 2016·Mycopathologia·G Sybren de HoogYvonne Gräser

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Citations

Jul 18, 2019·Expert Review of Proteomics·Martin WelkerDavid Pincus
Feb 2, 2021·Clinical, Cosmetic and Investigational Dermatology·Shambel ArayaAbebe Edao Negesso

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