Fast isolation of protein receptors from streptococci G by means of macroporous affinity discs

Journal of Chromatography. a
C KasperT B Tennikova

Abstract

A fast affinity method for the semi-preparative isolation of recombinant Protein G from E. coli cell lysate is proposed. Rigid, macroporous affinity discs based on a glycidyl methacrylate-co-ethylene dimethacrylate polymer were used as chromatographic supports. The specific ligands (here human immunoglobulin G, hIgG) were immobilized by the one-step reaction between native epoxy groups of the polymer surface and epsilon-amino groups of the IgG molecules. No intermediate spacer was necessary to reach full biological activity of the ligand. The globular affinity ligands are located directly on the pore wall surface and are thereby freely accessible to target molecules (here Protein G) migrating with the mobile phase through the pores. It is shown that the conditions chosen for the hIgG immobilization do not involve an active site of the protein and thus do not bias the formation of the affinity complex. Chromatographically determined constants of dissociation of hIgG-Protein G affinity complexes confirm the high selectivity of this separation method. Two different aspects of the affinity separation are discussed, which differ mostly in terms of scale. In disc chromatography, high volumetric flow velocities are possible because of...Continue Reading

References

Apr 24, 1992·Journal of Chromatography·M NachmanP Bailon
Jan 24, 1992·Journal of Chromatography·D JosićW Reutter
Feb 7, 1992·Journal of Chromatography·P Langlotz, K H Kroner
Oct 19, 1990·Journal of Chromatography·M UnarskaB J Bellhouse
Feb 11, 1994·Journal of Chromatography. a·J LuksaD Josic
Nov 15, 1996·Journal of Chromatography. a·N I DubininaT B Tennikova

❮ Previous
Next ❯

Citations

Aug 11, 2007·Analytical and Bioanalytical Chemistry·N DelmotteC G Huber
Sep 11, 2004·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Evgenia G VlakhTatiana B Tennikova
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·D Josić, A Buchacher
Nov 6, 2001·Journal of Biochemical and Biophysical Methods·H ZouD Zhou
Apr 14, 1999·Journal of Biotechnology·J HagedornT Tennikova
Dec 7, 2000·Journal of Pharmaceutical and Biomedical Analysis·L G BerruexT B Tennikova
May 16, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Natalia D OstryaninaTatiana B Tennikova
Jul 26, 2002·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Quanzhou LuoHanfa Zou
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova
Jul 30, 2004·Journal of Chromatography. a·Troii HallChristine E Smith
Mar 24, 2005·Journal of Chromatography. a·Galina A Platonova, Tatiana B Tennikova
Mar 24, 2005·Journal of Chromatography. a·Tatiana B Tennikova, Joachim Reusch
Jan 7, 2005·Journal of Peptide Science : an Official Publication of the European Peptide Society·E VlakhT Tennikova
Sep 15, 2006·Journal of Separation Science·Rangan Mallik, David S Hage
Oct 27, 2007·Journal of Separation Science·Evgenia G Vlakh, Tatiana B Tennikova
Jul 16, 2009·Journal of Separation Science·Galina A PlatonovaTatiana B Tennikova
Apr 4, 2021·Polymers·Evgenia Korzhikova-VlakhTatiana Tennikova
May 10, 2002·Journal of Chromatography. a·Natalia D OstryaninaTatiana B Tennikova
Oct 31, 2000·Analytical Chemistry·R Hahn, A Jungbauer
Nov 23, 2006·Journal of Chromatography. a·I V KalashnikovaT B Tennikova
Jan 11, 2000·Analytical Chemistry·A PodgornikT Koloini
Apr 15, 2005·Analytical Chemistry·Tao JiangDavid S Hage
Sep 3, 1998·Analytical Chemistry·R GiovanniniT B Tennikova
Aug 1, 1999·Analytical Chemistry·A PodgornikT Tennikova

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.