Fast migrating protein, immunochemically related to human factor VIII, studied by crossed immunoelectrophoresis in agarose

British Journal of Haematology
K B ThomasB G Firkin

Abstract

A fast migrating protein (FMP) was detected by agarose radio-crossed immunoelectrophoresis, in addition to factor VIII antigen (VIII:RAg), using antiserum to human factor VIII (FVIII). FMP had partial immunochemical identity with FVIII, migrated as an alpha-protein, and was distinct from alpha-2 macroglobulin, fibronectin or IgM. FMP was precipitated by concanavalin A and was separable from the bulk of VIII:RAg by ammonium sulphate fractionation. A significant amount of FMP was seen in normal serum (n = 12), plasma from patients with: (a) disseminated intravascular coagulation (n = 12) and (b) severe haemophilia A (n = 6). Trace amounts of FMP were observed in plasma from normal donors (n = 12), but neither VIII:ARg nor FMP was detectable in the plasma or serum from patients with severe von Willebrand's disease (n = 3). Freshly prepared cryoprecipitate contained trace amounts of FMP, similar to normal plasma, but increased levels were observed in antihaemophilic factor concentrates prepared for patient use. Significant levels of FMP were also seen in cryoprecipitate after storage at 4 degrees C for 7 d and this generation of FMP was diminished by the addition of protease inhibitors. The presence of significant levels of FMP in ...Continue Reading

References

Mar 1, 1977·Journal of Clinical Pathology·P Maisonneuve, Y Sultan
Jun 1, 1978·The Journal of Clinical Investigation·R R Montgomery, T S Zimmerman
Dec 1, 1978·The Journal of Clinical Investigation·J KouttsT S Zimmerman
Mar 10, 1979·The Medical Journal of Australia·M A HowardB G Firkin
Dec 13, 1979·The New England Journal of Medicine·T S ZimmermanD Meyer
Nov 1, 1977·British Journal of Haematology·J KouttsD Meyer
Jan 1, 1979·Applied Neurophysiology·J M DeniauD Albe-Fessard
Jan 1, 1975·The Journal of Pediatrics·G NathensonR Eisenberg
Jun 1, 1973·Australian and New Zealand Journal of Medicine·B FirkinL Stott
Mar 1, 1974·British Journal of Haematology·P B KernoffC R Rizza
Jul 18, 1974·The New England Journal of Medicine·I R PeakeJ C Giddings
Jan 1, 1974·Scandinavian Journal of Haematology·L Holmberg, I M Nilsson
Jul 1, 1980·Thrombosis Research·M A HowardJ Koutts
May 1, 1982·British Journal of Haematology·K B ThomasB G Firkin

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Citations

Jan 1, 1987·Thrombosis Research·T YamamotoB Blombäck
Sep 1, 1988·Blood Reviews·B G FirkinS M Jane
Mar 1, 1985·British Journal of Haematology·H H SalemB G Firkin
Nov 1, 1983·British Journal of Haematology·V VicenteA Lopez Borrasca
Jun 1, 1987·Australian and New Zealand Journal of Medicine·B G FirkinP Ryan

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