Fast model-free deconvolution of fluorescence decay for analysis of biological systems

Journal of Biomedical Optics
Javier A JoLaura Marcu

Abstract

For complex biological systems, conventional analysis of fluorescence intensity decay in terms of discrete exponential components cannot readily provide a true representation of the underlying fluorescence dynamics. We investigate an alternative nonparametric method for the analysis of time-resolved fluorescence data from biochemical and biological systems based on the expansion of fluorescence decay in a discrete Laguerre basis. We report that a unique Laguerre expansion can be found for fluorescence intensity decays of arbitrary form with convergence to a correct solution significantly faster than conventional multiexponential approximation methods. The Laguerre expansion coefficients are shown to be highly correlated with intrinsic fluorescence lifetimes and allow direct characterization of the fluorescence dynamics. A novel method for prediction of concentrations in mixtures of biochemical components using these coefficients is developed and successfully tested (prediction error <2%) using data from different mixtures of fluorescence lifetime standards. These findings suggest that the use of Laguerre expansion coefficients is a fast approach for the characterization and discrimination of complex biological systems such as t...Continue Reading

References

Jun 1, 1974·Analytical Biochemistry·A Grinvald, I Z Steinberg
Nov 1, 1993·Annals of Biomedical Engineering·V Z Marmarelis
Nov 1, 1993·Annals of Biomedical Engineering·V Z MarmarelisN H Holstein-Rathlou
Apr 7, 2007·Annals of Biomedical Engineering·Javier A JoMichael C K Khoo

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Citations

May 25, 2005·Annals of Biomedical Engineering·Olga V IvanovaMichael C K Khoo
Jan 26, 2012·Annals of Biomedical Engineering·Laura Marcu
May 25, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Jeremy D MeierD Gregory Farwell
Jun 15, 2006·Analytical Chemistry·Kristin A FletcherIsiah M Warner
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·William H YongLaura Marcu
Jan 18, 2006·Journal of Biomedical Optics·Pramod V ButteLaura Marcu
Mar 10, 2010·Journal of Biomedical Optics·Laura Marcu
May 13, 2010·Journal of Biomedical Optics·Pramod V ButteLaura Marcu
Nov 9, 2010·Journal of Biomedical Optics·Yinghua SunLaura Marcu
Nov 9, 2010·NeuroImage·Pramod V ButteLaura Marcu
May 28, 2008·Journal of Microscopy·V K RamanujanB A Herman
Dec 13, 2016·Journal of Biophotonics·Alix Le MaroisRainer Heintzmann
Jul 30, 2015·Journal of Biomedical Optics·Daniel U Campos-DelgadoJavier A Jo
Sep 2, 2004·Photochemistry and Photobiology·Laura MarcuReid C Thompson
Sep 15, 2015·Optics Express·Daniel U Campos-DelgadoJavier A Jo
Sep 16, 2020·Annals of Biomedical Engineering·Nelda Vazquez-PortalatinAlyssa Panitch
Apr 3, 2020·Optics Letters·Sucbei MoonYoungjae Won
Jun 6, 2013·Optics Express·Farzad FereidouniHans C Gerritsen

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