Fast Profiling of Natural Pigments in Different Spirulina (Arthrospira platensis) Dietary Supplements by DI-FT-ICR and Evaluation of their Antioxidant Potential by Pre-Column DPPH-UHPLC Assay

Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry
Eduardo SommellaPietro Campiglia

Abstract

Arthrospira platensis, better known as Spirulina, is one of the most important microalgae species. This cyanobacterium possesses a rich metabolite pattern, including high amounts of natural pigments. In this study, we applied a combined strategy based on Fourier Transform Ion Cyclotron Resonance Mass Spectrometry (FT-ICR-MS) and Ultra High-Performance Liquid Chromatography (UHPLC) for the qualitative/quantitative characterization of Spirulina pigments in three different commercial dietary supplements. FT-ICR was employed to elucidate the qualitative profile of Spirulina pigments, in both direct infusion mode (DIMS) and coupled to UHPLC. DIMS showed to be a very fast (4 min) and accurate (mass accuracy ≤ 0.01 ppm) tool. 51 pigments were tentatively identified. The profile revealed different classes, such as carotenes, xanthophylls and chlorophylls. Moreover, the antioxidant evaluation of the major compounds was assessed by pre-column reaction with the DPPH radical followed by fast UHPLC-PDA separation, highlighting the contribution of single analytes to the antioxidant potential of the entire pigment fraction. β-carotene, diadinoxanthin and diatoxanthin showed the highest scavenging activity. The method took 40 min per sample, c...Continue Reading

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Nov 7, 2016·Biotechnology Advances·Mengyue Gong, Amarjeet Bassi

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Citations

May 6, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Rita P AquinoFrancesca Sansone
Jul 10, 2019·Antioxidants·Mariosimone ZoccaliLuigi Mondello
Aug 23, 2020·Antioxidants·Francesco ManfellottoMaria Immacolata Ferrante
Nov 22, 2020·International Journal of Molecular Sciences·Albino CarrizzoCarmine Vecchione
May 1, 2021·Marine Drugs·Shaden A M KhalifaHesham R El-Seedi

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