Fast purification of thioredoxin reductases and of thioredoxins with an unusual redox-active centre from anaerobic, amino-acid-utilizing bacteria.

Microbiology
C HarmsJ R Andreesen

Abstract

Thioredoxin reductase and thioredoxin are primarily involved in catabolic metabolism as important electron carriers in anaerobic, amino-acid-degrading bacteria. A general and fast procedure was developed for the purification of thioredoxin reductase and thioredoxin from Eubacterium acidaminophilum, Clostridium litorale, C. sticklandii, C. sporogenes, C. cylindrosporum and 'Tissierella creatinophila' based upon their properties: the binding to 2',5'-AMP-Sepharose by thioredoxin reductase and the inability of thioredoxins to bind to a DEAE-Sephacel column. The consensus sequence at the active site of thioredoxins (-WCGPC-) was found to be modified in all of these anaerobes: Trp-31 (Escherichia coli nomenclature) was replaced by Gly or Ser, Gly-33 by Val or Glu. None of these thioredoxins reacted with thioredoxin reductase of E. coli or vice versa, but they did interact with the thioredoxin reductases obtained from the other anaerobes studied. Based upon their distinguishing features it is suggested that these thioredoxins might form an evolutionarily separate group.

References

Sep 1, 1978·Archives of Biochemistry and Biophysics·J I Elliott, J M Brewer
Jan 1, 1978·Methods in Enzymology·T C Stadtman
Dec 1, 1988·FEMS Microbiology Reviews·F K Gleason, A Holmgren
Jan 1, 1985·Annual Review of Biochemistry·A Holmgren
Dec 5, 1968·European Journal of Biochemistry·A Holmgren
Apr 1, 1983·Journal of Bacteriology·P Dürre, J R Andreesen
Jan 1, 1995·Methods in Enzymology·A Holmgren, M Björnstedt
Jan 1, 1994·Antonie van Leeuwenhoek·J R Andreesen
Apr 1, 1995·Archives of Microbiology·M Wagner, J R Andreesen
Nov 1, 1994·Archives of Biochemistry and Biophysics·B B BuchananR M Lozano
Oct 1, 1994·International Journal of Systematic Bacteriology·M D CollinsJ A Farrow

❮ Previous
Next ❯

Citations

Sep 14, 2010·Extremophiles : Life Under Extreme Conditions·Craig Baker-AustinMark Dopson
Nov 4, 2010·The Journal of Microbiology·Hee-Jeong Seo, Young Nam Lee
Mar 17, 2009·Journal of Bacteriology·Michael A ReottC Jeffrey Smith
Apr 7, 2015·Journal of Dairy Science·M A Aguinaga CasañasB Kuhla
Oct 30, 2016·The Journal of Biological Chemistry·Dwi SusantiBiswarup Mukhopadhyay

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.