Fast rewarming after deep hypothermic circulatory arrest in rats impairs histologic outcome and increases NFκB expression in the brain

Perfusion
M Lucia GordanBettina Jungwirth

Abstract

Deep hypothermia is used as a neuroprotectant during cardiac surgery utilizing deep hypothermic circulatory arrest (DHCA), although the ideal rewarming strategy is not known yet. Some of the neuroprotective properties of hypothermia seem to be mediated by Nuclear Factor Kappa B (NFκB) as an important transcription factor. The current study was designed to investigate the effect of the rewarming rate on histologic outcome and cerebral NFκB expression one day following DHCA in rats. With IRB approval, 20 rats were cannulated for cardiopulmonary bypass (CPB), cooled to a rectal temperature of 15-18°C, subjected to 45min of DHCA and randomly assigned to either a slow (40 min) or a fast (20 min) rewarming protocol. At 24 hours post DHCA, the number of eosinophilic neurons was analyzed with hematoxylin and eosin (HE) staining, and NFκB expression immunohistochemically. The two experimental groups were compared with untreated control rats. HE staining showed more eosinophilic neurons in the motor cortex following fast rewarming (60 [15-388]) compared to slow rewarming (15 [10-21]) (p<0.05). Neuronal expression of NFκB was increased in the fast rewarming group in both brain areas, the motor cortex (fast: 258 [135-393]; slow: 165 [80-21...Continue Reading

References

May 3, 2002·The Journal of Thoracic and Cardiovascular Surgery·Joseph S Coselli, Scott A LeMaire
Apr 21, 2004·Drug News & Perspectives·Timothy C Nichols
Apr 4, 2006·The Journal of Thoracic and Cardiovascular Surgery·Bettina JungwirthGeorg Nollert
Jun 6, 2006·Neurochemistry International·Midori A Yenari, Hyung Soo Han
Dec 10, 2008·Neurobiology of Disease·Carla M WebsterMidori A Yenari

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Citations

Oct 9, 2012·Annals of Cardiac Anaesthesia·Michael MazzeffiGregory Fischer
Jun 6, 2012·Resuscitation·Kjetil Sunde
Nov 15, 2011·Resuscitation·Jakobea Benz-WoernerMauro Oddo
Jun 15, 2012·Journal of Veterinary Emergency and Critical Care·Daniel J FletcherUNKNOWN Veterinary Emergency and Critical Care Society
Sep 18, 2012·Journal of Cardiac Surgery·Qun GuShijiang Zhang
Jan 27, 2015·Journal of Cardiothoracic and Vascular Anesthesia·Michael SecoMichael P Vallely
Dec 31, 2014·Clinical and Experimental Emergency Medicine·You Hwan JoIn Soo Cho
Jan 6, 2016·Academic Emergency Medicine : Official Journal of the Society for Academic Emergency Medicine·Guillaume DebatyRaphaël Briot
May 15, 2020·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Daniele LinardiAlessio Rungatscher
Apr 25, 2017·Therapeutic Hypothermia and Temperature Management·Alessio RungatscherGiuseppe Faggian
Apr 23, 2020·Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine·Aurélien Ledoux, Piehr Saint Leger

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