Fast RPLC-UV method on short sub-two microns particles packed column for the assay of tenoxicam in plasma samples

Journal of Pharmaceutical and Biomedical Analysis
Iulia SoraAndrei Medvedovici

Abstract

An extraction-less sample preparation technique followed by a RPLC-UV method on sub-two microns particles packed short column were used for the assay of tenoxicam in plasma samples. Protein precipitation was made by means of trichloroacetic acid addition. Supernatant was injected to the chromatographic column without any further pH adjustment. The mobile phase consisted in a mixture of acetonitrile and aqueous 0.1% phosphoric acid, at 2 mL/min flow rate and gradient elution. The Zorbax SB-C18 column (50 mm length, 4.6 mm internal diameter and 1.8 microm particle size) was thermostated at 60 degrees C. The mobile phase gradient composition program allowed separation of tenoxicam and piroxicam (internal standard), column clean-up and re-equilibration within 4 min. UV detection was achieved at 368+/-10 nm. The method is characterized by a low limit of quantitation of 25 ng/mL for tenoxicam, with a linearity interval up to 5500 ng/mL. The use of a low volume detection cell and detector high frequency data acquisition rate produced high precision and accuracy through a whole bioequivalence study of tenoxicam in two commercially available tablet formulations, after a single oral administration dose. Full method validation is presente...Continue Reading

References

Jan 1, 1989·Fundamental & Clinical Pharmacology·F BreeJ P Tillement
Jan 1, 1989·European Journal of Drug Metabolism and Pharmacokinetics·C CrevoisierU C Dubach
Oct 1, 1988·British Journal of Clinical Pharmacology·R O DayD N Wade
Jan 1, 1988·European Journal of Clinical Pharmacology·O G NilsenE Thue
Jan 10, 1986·Journal of Chromatography·P HeizmannK Zinapold
Jan 1, 1987·Scandinavian Journal of Rheumatology. Supplement·H Fenner
Mar 24, 1995·Journal of Chromatography. B, Biomedical Applications·J L Mason, G J Hobbs
Jun 1, 1994·Journal of Clinical Pharmacy and Therapeutics·M S SalemA A Badwan
Jan 1, 1994·Clinical Pharmacokinetics·O G Nilsen
Feb 1, 1994·Clinical Pharmacokinetics·K T OlkkolaM J Mattila
Aug 1, 1993·Biopharmaceutics & Drug Disposition·T Stebler, T W Guentert
Jul 2, 1993·Journal of Chromatography·M I Múnera-Jaramillo, S Botero-Garcés
Jan 6, 2001·Journal of Chromatographic Science·J J Kirkland
May 14, 2003·Journal of Chromatography. a·Yanqiao XiangMilton L Lee
Jun 18, 2005·Clinical Pharmacology and Therapeutics·Guilherme Suarez-KurtzSergio D J Pena
Sep 20, 2005·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hye Young JiHye Suk Lee
Oct 1, 2005·Journal of Chromatography. a·Michel Martin, Georges Guiochon

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

© 2021 Meta ULC. All rights reserved