Fast separation of triterpenoid saponins using supercritical fluid chromatography coupled with single quadrupole mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Yang HuangZhengjin Jiang

Abstract

Triterpenoid saponins (TSs) are the most important components of some traditional Chinese medicines (TCMs) and have exhibited valuable pharmacological properties. In this study, a rapid and efficient method was developed for the separation of kudinosides, stauntosides and ginsenosides using supercritical fluid chromatography coupled with single quadrupole mass spectrometry (SFC-MS). The separation conditions for the selected TSs were carefully optimized after the initial screening of eight stationary phases. The best compromise for all compounds in terms of chromatographic performance and MS sensitivity was obtained when water (5-10%) and formic acid (0.05%) were added to the supercritical carbon dioxide/MeOH mobile phase. Beside the composition of the mobile phase, the nature of the make-up solvent for interfacing SFC with MS was also evaluated. Compared to reversed phase liquid chromatography, the SFC approach showed higher resolution and shorter running time. The developed SFC-MS methods were successfully applied to the separation and identification of TSs present in Ilex latifolia Thunb., Panax quinquefolius L. and Panax ginseng C.A. Meyer. These results suggest that this SFC-MS approach could be employed as a useful tool f...Continue Reading

References

Dec 30, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·M TuomolaP Manninen
Jan 5, 2002·Journal of Chromatography. a·C TurneL Mathiasson
Apr 18, 2006·Journal of Chromatography. a·Hongdeng QiuLiang Zhao
Apr 28, 2009·Journal of Pharmaceutical and Biomedical Analysis·Bai-Shen SunChang-Keun Sung
Jun 27, 2009·Archives of Pharmacal Research·Ju Hyeon LeeYoumie Park
Apr 22, 2014·Journal of Ginseng Research·Sena LeeYoun-Jung Kim
Jan 22, 2015·Journal of Chromatography. a·Eric Lesellier, Caroline West
Nov 21, 2015·Journal of Pharmaceutical and Biomedical Analysis·Canjian WangLiang Liu

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Citations

Oct 17, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yang HuangTiejie Wang
Sep 2, 2017·Journal of Separation Science·Congcong XuTong Zhang
Jul 28, 2018·Analytical and Bioanalytical Chemistry·Caroline West

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