Fast structural changes (200-900ns) may prepare the photosynthetic manganese complex for oxidation by the adjacent tyrosine radical

Biochimica Et Biophysica Acta
André KlaussH Dau

Abstract

The Mn complex of photosystem II (PSII) cycles through 4 semi-stable states (S(0) to S(3)). Laser-flash excitation of PSII in the S(2) or S(3) state induces processes with time constants around 350ns, which have been assigned previously to energetic relaxation of the oxidized tyrosine (Y(Z)(ox)). Herein we report monitoring of these processes in the time domain of hundreds of nanoseconds by photoacoustic (or 'optoacoustic') experiments involving pressure-wave detection after excitation of PSII membrane particles by ns-laser flashes. We find that specifically for excitation of PSII in the S(2) state, nuclear rearrangements are induced which amount to a contraction of PSII by at least 30Å(3) (time constant of 350ns at 25°C; activation energy of 285+/-50meV). In the S(3) state, the 350-ns-contraction is about 5 times smaller whereas in S(0) and S(1), no volume changes are detectable in this time domain. It is proposed that the classical S(2)=>S(3) transition of the Mn complex is a multi-step process. The first step after Y(Z)(ox) formation involves a fast nuclear rearrangement of the Mn complex and its protein-water environment (~350ns), which may serve a dual role: (1) The Mn- complex entity is prepared for the subsequent proton ...Continue Reading

References

Jan 1, 1992·Biophysical Chemistry·J R SmallE W Small
May 25, 1972·Biochimica Et Biophysica Acta·J B CallisM Gouterman
May 9, 1980·Biochimica Et Biophysica Acta·H Conjeaud, P Mathis
Apr 27, 1999·Biochimica Et Biophysica Acta·M Haumann, W Junge
Dec 15, 2000·Biochimica Et Biophysica Acta·J H RobbleeV K Yachandra
Dec 15, 2000·Biochimica Et Biophysica Acta·F Rappaport, J Lavergne
Jun 26, 2002·Biochemistry·Fabrice RappaportJérôme Lavergne
Nov 20, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Wolfgang JungeJürgen Clausen
Feb 7, 2004·Science·Kristina N FerreiraSo Iwata
Jan 18, 2005·Biophysical Journal·Fabrice RappaportJérôme Lavergne
Jul 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·O CanaaniD Mauzerall
Apr 28, 2006·Proceedings of the National Academy of Sciences of the United States of America·A R HolzwarthM Rögner
Nov 10, 2006·Chemical Reviews·James P McEvoy, Gary W Brudvig
Apr 3, 2007·Biochimica Et Biophysica Acta·Roland KrivanekMichael Haumann
Nov 9, 2007·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Holger DauMichael Haumann
Feb 7, 2008·Physiologia Plantarum·Markus Grabolle, Holger Dau
Feb 23, 2008·Journal of the American Chemical Society·Eduardo M SprovieroVictor S Batista
Apr 12, 2008·The Journal of Physical Chemistry. a·Alberto C RizziSilvia E Braslavsky
Aug 6, 2008·Chemistry : a European Journal·Per E M Siegbahn
Oct 3, 2008·Photosynthesis Research·Gernot Renger, Thomas Renger
Feb 17, 2009·Nature Structural & Molecular Biology·Albert GuskovWolfram Saenger

❮ Previous
Next ❯

Citations

Jun 4, 2014·Proceedings of the National Academy of Sciences of the United States of America·Daniele NarziLeonardo Guidoni
Dec 7, 2019·Physical Chemistry Chemical Physics : PCCP·Shin NakamuraLeonardo Guidoni
Jun 13, 2019·Photosynthesis Research·Wolfgang LubitzNicholas Cox
May 3, 2020·Physiologia Plantarum·Miwa SugiuraAlain Boussac
Mar 27, 2020·Annual Review of Biochemistry·Nicholas CoxWolfgang Lubitz
Feb 18, 2020·Biochimica Et Biophysica Acta. Bioenergetics·Alain BoussacMiwa Sugiura

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.