Fast track, dynein-dependent nuclear targeting of human immunodeficiency virus Vpr protein; impaired trafficking in a clinical isolate

Biochemical and Biophysical Research Communications
Leon CalyDavid A Jans

Abstract

Nuclear import of the accessory protein Vpr is central to infection by human immunodeficiency virus (HIV). We previously identified the Vpr F72L mutation in a HIV-infected, long-term non-progressor, showing that it resulted in reduced Vpr nuclear accumulation and altered cytoplasmic localisation. Here we demonstrate for the first time that the effects of nuclear accumulation of the F72L mutation are due to impairment of microtubule-dependent-enhancement of Vpr nuclear import. We use high resolution imaging approaches including fluorescence recovery after photobleaching and other approaches to document interaction between Vpr and the dynein light chain protein, DYNLT1, and impaired interaction of the F72L mutant with DYNLT1. The results implicate MTs/DYNLT1 as drivers of Vpr nuclear import and HIV infection, with important therapeutic implications.

References

Feb 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·P BouchardC W Bardin
Nov 17, 1998·The Journal of Cell Biology·Y JenkinsW C Greene
Jan 20, 2001·The Journal of Biological Chemistry·Y K MokM Zhang
Jan 11, 2001·Journal of Virology·M P ShermanW C Greene
Jan 31, 2002·Molecular Endocrinology·Mark H C LamDavid A Jans
Nov 6, 2002·The Journal of Cell Biology·David McDonaldThomas J Hope
Dec 12, 2002·Biochemistry·Russell J DiefenbachAnthony L Cunningham
May 24, 2003·Proceedings of the National Academy of Sciences of the United States of America·Vincent R HarleyDavid A Jans
Jun 21, 2003·Current Drug Targets·S C PillerD A Jans
Jul 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·Melike LakadamyaliXiaowei Zhuang
May 15, 2004·DNA and Cell Biology·Shohreh AminiBassel E Sawaya
May 26, 2004·AIDS Research and Human Retroviruses·Angela CiuffiPascal R A Meylan
Feb 24, 2005·Retrovirology·Erwann Le Rouzic, Serge Benichou
Sep 23, 2006·Nature Methods·Nathalie ArhelPierre Charneau
Dec 5, 2006·PLoS Pathogens·Joshua L AndersenVicente Planelles
May 22, 2007·Traffic·Daniela Martino RothDavid A Jans
Jun 15, 2007·Molecular Biology of the Cell·Gregory W MoseleyDavid A Jans
Oct 9, 2007·Biochemical and Biophysical Research Communications·Danielle Renee FontenotRakesh Kumar
Jul 24, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jirí VlachTomás Ruml
Dec 31, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alex J FulcherDavid A Jans
Jul 20, 2010·Current Opinion in Biotechnology·Ming Je HsiehColin W Pouton
Feb 23, 2011·The Journal of Biological Chemistry·Daniela Martino RothDavid A Jans
Feb 19, 2013·Cell Host & Microbe·Sofia V ZaichickGregory A Smith
May 14, 2014·The Journal of Cell Biology·Kim G LieuDavid A Jans
Oct 28, 2015·The Journal of Cell Biology·Audrey DumasFlorence Niedergang

❮ Previous
Next ❯

Citations

Jun 24, 2017·Cellular Microbiology·Gloria Arriagada
Nov 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Adarsh DharanEdward M Campbell
Jun 15, 2018·Journal of Virology·Adarsh Dharan, Edward M Campbell

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
Y Kawasak
Methods in Molecular Biology
J DélèzeJ C Hervé
Focus : a Guide to AIDS Research
J Grimshaw
© 2022 Meta ULC. All rights reserved