Fast x-ray recordings reveal dynamic action of contractile and regulatory proteins in stretch-activated insect flight muscle.

Biophysical Journal
Hiroyuki IwamotoNaoto Yagi

Abstract

To assess the ability of the thin-filament regulatory system to control each stretch-activation (SA) event in the fast beating of asynchronous insect flight muscle (IFM), we obtained fast (3.4 ms/frame) and semistatic (> or = 50 ms) x-ray diffraction recordings for IFM fibers from bumblebees (beating at 170 Hz) and compared the results with those acquired in giant waterbugs (20-30 Hz) and crane flies (40 Hz, semistatic only). In contrast to the well-documented large SA force of waterbug IFMs, the SA force of bumblebee and crane fly IFMs was small compared to their large isometric force. In semistatic recordings, step-stretched bumblebee and crane fly IFMs showed smaller net SA-associated intensity changes in reflections that report myosin attachment to actin and tropomyosin movement toward its activating position. However, fast recordings on bumblebee IFMs showed a fast and large temporary reversal of intensities in these reflections, suggesting that the myosin heads supporting isometric force are dynamically replaced by SA-supporting heads, and that tropomyosin moves to and back from its inactivating position in milliseconds. In waterbug IFMs, the fast temporary reversal of intensities was not obvious. The observed rates of th...Continue Reading

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Citations

Mar 15, 2013·Journal of the Royal Society, Interface·Fritz-Olaf LehmannRuben Berthé
Aug 24, 2013·Science·H Iwamoto, N Yagi
Dec 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Robert J Perz-EdwardsMichael K Reedy
Aug 20, 2014·Journal of Muscle Research and Cell Motility·Catherine C EldredDouglas M Swank
Jan 8, 2013·Biochemical and Biophysical Research Communications·Hiroyuki Iwamoto
Apr 17, 2012·Journal of Structural Biology·Masaya NishiuraHiroyuki Iwamoto
Nov 10, 2011·Biophysical Journal·Qian WangDouglas M Swank
Sep 23, 2016·Biophysical Journal·Toshiki ShimomuraMadoka Suzuki
Sep 12, 2018·International Journal of Molecular Sciences·Weikang MaThomas Irving
Aug 25, 2017·American Journal of Physiology. Cell Physiology·Bernadette M GlasheenDouglas M Swank
Feb 17, 2011·Biophysics·Hiroyuki Iwamoto
Jun 15, 2018·International Journal of Molecular Sciences·Hiroyuki Iwamoto

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