Fasudil may induce the differentiation of bone marrow mesenchymal stem cells into neuron‑like cells via the Wnt/β‑catenin pathway

Molecular Medicine Reports
Yahui HuWei Lu

Abstract

Bone mesenchymal stem cells (MSCs) are an excellent donor graft source due to their potential for self‑renewal and multidirectional differentiation. However, it is difficult to obtain high quality MSCs and to induce them to differentiate into neuron‑like cells. Fasudil, a Rho kinase inhibitor, exhibits therapeutic potential in spinal cord injuries and stroke. The present study investigated the effect of fasudil on the differentiation of MSCs into neuron‑like cells. MSCs were obtained from rat femur marrow, expanded in culture medium, and used at the third passage for subsequent experiments. MSCs were pre‑induced with 10 ng/ml basic fibroblast growth factor (bFGF) for 24 h, which was followed by induction with fasudil. A control untreated group and a group treated with fasudil + XAV939, a Wnt/β‑catenin pathway inhibitor, were also used in the present study. Reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), western blot analysis and immunofluorescence staining were performed in order to detect neuron‑specific markers, including neuron‑specific enolase (NSE), nestin and neurofilament‑M (NF‑M). Following induction with fasudil, neuron‑like cell morphology was observed. In the fasudil + XAV939 and control group...Continue Reading

References

Apr 15, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A Eglitis, E Mezey
May 15, 1999·Science·B E PetersenJ P Goff
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·G C KopenD G Phinney
Feb 17, 2001·Current Opinion in Neurobiology·B J Dickson
Mar 22, 2001·Nature Reviews. Neuroscience·L Luo
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Oct 24, 2002·Neurological Research·Pedro CuevasDiana Reimers
Oct 14, 2003·Nature·Manuel Alvarez-DoladoArturo Alvarez-Buylla
Sep 15, 2004·Experimental & Molecular Medicine·Rokeya BegumM A Zaman
Feb 24, 2005·Stem Cells and Development·Xiaoxiao LongMorris Kletzel
Mar 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Takako KondoEri Hashino
Aug 2, 2005·International Journal of Oral and Maxillofacial Surgery·B-H ChoiJ-H Jung
Oct 13, 2006·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Hwa Kyoung ShinCenk Ayata
Jun 19, 2007·Biochemical and Biophysical Research Communications·Satoshi ShimizuMari Dezawa
Jun 28, 2007·Neurochemical Research·Jun-ichi Sagara, Nobuo Makino
Mar 29, 2008·Methods in Molecular Biology·Shijie Song, Juan Sanchez-Ramos
Apr 10, 2008·Cell Stem Cell·Paolo BiancoPaul J Simmons
Sep 12, 2008·Cellular and Molecular Neurobiology·Qin LiLian-Jun Guo
Oct 14, 2008·Biochemical and Biophysical Research Communications·Emilie PacaryMyriam Bernaudin
Feb 16, 2011·Journal of Cell Science·Soon-Tuck Sit, Ed Manser
Apr 14, 2011·Yonsei Medical Journal·Keum Seok BaeSeong Joon Kang
Jun 27, 2012·The Journal of Pharmacology and Experimental Therapeutics·Daniela ImpellizzeriSalvatore Cuzzocrea
Oct 4, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Shuyang WangYanjie Jia
Aug 21, 2013·Biochemical and Biophysical Research Communications·Qin YuWei Liu

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Citations

Mar 27, 2021·Canadian Journal of Physiology and Pharmacology·Rukiye Nalan TiftikSeyhan Şahan-Fırat

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Methods Mentioned

BETA
PCR
flow cytometry
transfection

Software Mentioned

WinMD I
GraphPad Prism
GraphPad

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