fat-1 transgenic zebrafish are protected from abnormal lipid deposition induced by high-vegetable oil feeding

Applied Microbiology and Biotechnology
Shouxiang SunJian Gao

Abstract

High dietary concentration of vegetable oil, particularly those rich in n-6 polyunsaturated fatty acids (PUFAs), can induce negative physiological effects including excessive lipid deposition in teleost fish. Omega-3 desaturase (Fat-1) of Caenorhabditis elegans is able to convert n-6 PUFAs to n-3 PUFAs and thus induces a low n-6/n-3 PUFAs ratio alleviating lipid deposition. In this study, we investigated the effects of dietary n-6 PUFAs on lipid metabolism of fat-1 transgenic zebrafish (Tg:fat-1), to explore the role of fat-1 in fish lipid metabolism. We first generated Tg:fat-1 zebrafish and assayed the effects of a low-fat diet (LFD) and a high-fat diet (HFD) prepared from soybean oil. Wild type zebrafish (WT) fed with HFD (HFD-WT) exhibited increased obesity and lipid deposition, especially in the abdominal cavity and liver. These defects were absent from HFD-Tg:fat-1. For each diet group, Tg:fat-1 exhibited significantly decreased levels of almost all hepatic lipid classes compared with WT. Expression levels of lipid synthesis-related genes and lipid deposition-related genes were markedly lower in the liver of HFD-Tg:fat-1 compared with HFD-WT. In contrast, the steatolysis-related genes significantly upregulated in HFD-Tg:f...Continue Reading

References

Jan 1, 1985·Progress in Lipid Research·A M BeenakkersW J Van Marrewijk
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J P SpychallaJ Browse
Aug 19, 1999·Occupational Medicine·R M Niven, C A Pickering
Jan 10, 2002·Nature·Karim S EchtayMartin D Brand
May 8, 2002·The Journal of Clinical Investigation·Jay D HortonMichael S Brown
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Feb 7, 2004·Nature·Jing X KangZhao B Kang
Aug 1, 2006·Neuroscience Research·Jyhi-Wai WangYu-Ten Ju
May 15, 2008·Journal of Animal Physiology and Animal Nutrition·P NguyenH Dumon
Aug 18, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·John BostockRichard Corner
Oct 12, 2010·Biochimica Et Biophysica Acta·Richard C Scarpulla
Jan 19, 2012·American Journal of Physiology. Endocrinology and Metabolism·Maria H HolmströmPablo M Garcia-Roves
Nov 22, 2012·Best Practice & Research. Clinical Endocrinology & Metabolism·Laura D OsellameMichael R Duchen
Apr 24, 2015·Frontiers in Physiology·Rosalba PuttiLillà Lionetti
Mar 23, 2018·G3 : Genes - Genomes - Genetics·Mengjing LiDaxin Pang

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
light microscopy
electrophoresis

Software Mentioned

Excel
SPSS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.