Fat removal during cell salvage: an optimized program for a discontinuous autotransfusion device

Transfusion
Timo F SeyfriedErnil Hansen

Abstract

Fat in wound blood observed in orthopedic or cardiac surgery might pose a risk for fat embolism during blood salvage. Fat removal was optimized in the washing process. In an experimental study blood from fresh donations was adjusted to a hematocrit (Hct) of 25% and an admixture of 1.25% human tissue fat. This blood was processed with the cell salvage device XTRA in a modified program mode. Volumetric quantification of fat was performed after centrifugation of blood samples in Pasteur pipettes. From the volumes, the Hct levels and the concentrations of fat and other variables elimination rates and RBC recovery were calculated. Pretests showed wash volume, wash flow, and process interruptions affecting fat elimination. With the new optimized fat elimination program Pfat removal rate of fat increased to 98.5 ± 0.9% for the 225-mL bowl. The product had a mean Hct of 48.7 ± 1.2% and a RBC recovery rate of 93.5 ± 2.3%. The program conserved the high elimination rates for albumin, heparin, potassium, and free plasma hemoglobin (98.8, 99.3, 95.3, and 94.9%, respectively). Similar high fat removal was also observed with bowls of smaller size, namely, 98.1% for the 175-mL bowl and 98.2% for the 125- and the 55-mL bowls. With test blood o...Continue Reading

References

Nov 14, 1997·Anesthesia and Analgesia·M BookeH Van Aken
Feb 24, 1999·Journal of Neuropathology and Experimental Neurology·W R BrownV R Challa
Nov 18, 2000·The Annals of Thoracic Surgery·E H KincaidJ W Hammon
Feb 13, 2001·Anesthesia and Analgesia·M BookeF Hinder
Jun 20, 2002·Transfusion Medicine·J F BurmanJ R Pepper
Apr 16, 2003·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Anita E JewellGraham Cooper
Aug 18, 2012·Transfusion·Michael GruberErnil Hansen
Jun 24, 2014·Transfusion·Timo SeyfriedErnil Hansen

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