Fat2 acts through the WAVE regulatory complex to drive collective cell migration during tissue rotation

The Journal of Cell Biology
Anna Julia SquarrSven Bogdan

Abstract

Directional cell movements during morphogenesis require the coordinated interplay between membrane receptors and the actin cytoskeleton. The WAVE regulatory complex (WRC) is a conserved actin regulator. Here, we found that the atypical cadherin Fat2 recruits the WRC to basal membranes of tricellular contacts where a new type of planar-polarized whip-like actin protrusion is formed. Loss of either Fat2 function or its interaction with the WRC disrupts tricellular protrusions and results in the formation of nonpolarized filopodia. We provide further evidence for a molecular network in which the receptor tyrosine phosphatase Dlar interacts with the WRC to couple the extracellular matrix, the membrane, and the actin cytoskeleton during egg elongation. Our data uncover a mechanism by which polarity information can be transduced from a membrane receptor to a key actin regulator to control collective follicle cell migration during egg elongation. 4D-live imaging of rotating MCF10A mammary acini further suggests an evolutionary conserved mechanism driving rotational motions in epithelial morphogenesis.

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Citations

Feb 24, 2016·The Journal of Cell Biology·Tomke Stürner, Gaia Tavosanis
Jun 25, 2017·Journal of Cell Science·Olga AlekhinaDaniel D Billadeau
Oct 19, 2017·Development·Julien ChlastaMuriel Grammont
May 10, 2019·Cell Cycle·Tara M Finegan, Dan T Bergstralh
Sep 7, 2019·Proceedings of the National Academy of Sciences of the United States of America·Yang GaoQian Li
Jul 31, 2020·Proceedings of the National Academy of Sciences of the United States of America·Maria BroounHelen McNeill
Sep 5, 2020·The Journal of Cell Biology·Masayuki OzawaMasatoshi Takeichi
Nov 16, 2018·Proceedings of the National Academy of Sciences of the United States of America·Amanda S ChinLeo Q Wan
Jul 11, 2020·Science Immunology·Elisabeth SalzerKaan Boztug
Oct 22, 2018·Neurogenetics·Júlia Canet-PonsUwe Warnken
Feb 6, 2021·Nature Communications·Maik C BischoffSven Bogdan
Sep 5, 2021·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Maik C Bischoff, Sven Bogdan

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Methods Mentioned

BETA
transgenic
pull down
pull-down
PCR
FCS
Transfection

Software Mentioned

Fiji ( ImageJ
Fiji
Zen
OrientationJ
Imaris
GraphPad
PRISM
LifeAct

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