Fatal intoxications by acenocoumarol, phenprocoumon and warfarin: method validation in blood using the total error approach

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Raphaël DenoozCorinne Charlier

Abstract

A simple high-performance liquid chromatography (HPLC) method with ultraviolet (UV) detection has been developed and validated for simultaneous identification and quantification of three antivitamin K drugs (acenocoumarol, warfarin and phenprocoumon) in whole blood. The aim of this development was to propose an analytical technique adapted to the situations of forensic toxicology, i.e. intoxication with massive anticoagulant doses, when the usual coagulation tests could not be used. The blood sample, after spiked with prazepam as an internal standard (IS), was submitted to a liquid-liquid extraction (LLE) prior to HPLC analysis. A chromatographic separation was achieved on a C8 Symmetry column with a mobile phase consisting of an acetonitrile and phosphate buffer (pH 3.8) mixture in a gradient mode. Detection was carried out at a wavelength between 200 and 400 nm. This method has been validated with the concept of total error as decision criterion. Trueness ranged from 99.1% to 105.0% and precision was good with RSD between 1.3% and 6.7%. Consequently, this rapid and simple chromatographic technique is well adapted to focus intoxications with most important coumarinic drugs available on pharmaceutical market and is now routinel...Continue Reading

Citations

Sep 26, 2009·European Journal of Clinical Pharmacology·Terhi LauniainenIlkka Ojanperä
Dec 17, 2011·Journal of Pharmaceutical and Biomedical Analysis·Mohammadreza Hadjmohammadi, Hoda Ghambari
May 25, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Hoda Ghambari, Mohammadreza Hadjmohammadi
Jan 13, 2015·Materials Science & Engineering. C, Materials for Biological Applications·Mohammad Bagher GholivandEilnaz Yavari
Sep 9, 2020·Critical Reviews in Analytical Chemistry·Suraj R ChaudhariAtul A Shirkhedkar
Aug 21, 2018·Biosensors & Bioelectronics·E Cristina PeláezLaura M Lechuga

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