Fate of bone marrow mesenchymal stromal cells following autologous transplantation in a rabbit model of osteonecrosis

Cytotherapy
Hisashi SugayaMasashi Yamazaki

Abstract

Internalizing quantum dots (i-QDs) are a useful tool for tracking cells in vivo in models of tissue regeneration. We previously synthesized i-QDs by conjugating QDs with a unique internalizing antibody against a heat shock protein 70 family stress chaperone. In the present study, i-QDs were used to label rabbit mesenchymal stromal cells (MSCs) that were then transplanted into rabbits to assess differentiation potential in an osteonecrosis model. The i-QDs were taken up by bone marrow-derived MSCs collected from the iliac of 12-week-old Japanese white rabbits that were positive for cluster of differentiation (CD)81 and negative for CD34 and human leukocyte antigen DR. The average rate of i-QD internalization was 93.3%. At 4, 8, 12, and 24 weeks after transplantation, tissue repair was evaluated histologically and by epifluorescence and electron microscopy. The i-QDs were detected at the margins of the drill holes and in the necrotized bone trabecular. There was significant colocalization of the i-QD signal in transplanted cells and markers of osteoblast and mineralization at 4, 8, and 12 weeks post-transplantation, while i-QDs were detected in areas of mineralization at 12 and 24 weeks post-transplantation. Moreover, i-QDs were ...Continue Reading

References

Jan 1, 1989·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·H OhgushiA I Caplan
Sep 1, 1988·The American Journal of Anatomy·S C Marks, S N Popoff
Apr 2, 1999·Science·M F PittengerD R Marshak
Jan 22, 2004·Bioconjugate Chemistry·Byron BallouAlan S Waggoner
Jun 2, 2005·Nature Materials·Igor L MedintzHedi Mattoussi
Jul 5, 2005·The Journal of Bone and Joint Surgery. American Volume·Ph HernigouH Rouard
Nov 28, 2006·British Journal of Sports Medicine·S J Warden
Apr 28, 2007·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Zeenia KaulRenu Wadhwa
Oct 18, 2007·Journal of Shoulder and Elbow Surgery·Ranjan Gupta, Thay Q Lee
Oct 18, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Zuoqin YanZhengrong Chen
Jun 19, 2010·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Takeshi OgawaNaoyuki Ochiai
Aug 28, 2010·Journal of Tissue Engineering and Regenerative Medicine·Tomokazu YoshiokaToshimasa Uemura
Mar 2, 2012·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ami R AminiSyam P Nukavarapu
May 3, 2012·Stem Cells International·Andreas SchmittStefan Buchmann
Oct 24, 2013·In Vitro Cellular & Developmental Biology. Animal·A BakhtinaT Livingston Arinzeh
Dec 12, 2013·BMC Research Notes·Tsung-Han LeeTao-Chen Lee
Nov 8, 2014·PloS One·Tao-Chen LeeMartin Hsiu-Chu Lin

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Citations

Dec 22, 2019·Applied Biochemistry and Biotechnology·Xueyan HuKedong Song
Aug 25, 2018·BMC Veterinary Research·Randolph L WinterAnne A Wooldridge
Nov 20, 2018·Dental Materials Journal·Guodong SongXiaolei Jin
May 11, 2018·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroshi Yukawa, Yoshinobu Baba

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