Fate of distal lung epithelium cultured in a decellularized lung extracellular matrix

Tissue Engineering. Part a
Elizabeth A CalleLaura E Niklason

Abstract

Type II cells are the defenders of the alveolus. They produce surfactant to prevent alveolar collapse, they actively transport water to prevent filling of the air sacs that would otherwise prevent gas exchange, and they differentiate to type I epithelial cells. They are an indispensable component of functional lung tissue. To understand the functionality of type II cells in isolation, we sought to track their fate in decellularized matrices and to assess their ability to contribute to barrier function by differentiation to type I alveolar epithelial cells. Rat type II cells were isolated from neonatal rat lungs by labeling with the RTII-70 surface marker and separation using a magnetic column. This produced a population of ∼50% RTII-70-positive cells accompanied by few type I epithelial cells or α-actin-positive mesenchymal cells. This population was seeded into decellularized rat lung matrices and cultured for 1 or 7 days. Culture in Dulbecco's modified Eagle's medium +10% fetal bovine serum (FBS) resulted in reduced expression of epithelial markers and increased expression of mesenchymal markers. By 7 days, no epithelial markers were visible by immunostaining; nearly all cells were α-actin positive. Gene expression for the me...Continue Reading

References

Jun 1, 1986·Thorax·G J Laurent
Aug 1, 1997·The American Journal of Physiology·L M Coluccio
Jul 13, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ward R RiceTimothy E Weaver
Jan 30, 2004·Annual Review of Medicine·Victor J ThannickalFernando J Martinez
Sep 28, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Robert GonzalezLeland Dobbs
Aug 30, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Nobuhiro IzumiKazuo Ikeda
Apr 4, 2006·Tissue Engineering·Bryan BrownStephen F Badylak
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kevin K KimHarold A Chapman
Jan 18, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zhaodi Gong, Laura E Niklason
Dec 30, 2008·PloS One·Lynne A MurrayDarryl A Knight
Jan 13, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Stephen P AroldBéla Suki
Feb 18, 2009·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Susan E WertLawrence M Nogee
Mar 20, 2010·Tissue Engineering. Part a·Andrew P PriceAngela Panoskaltsis-Mortari
Apr 23, 2010·Tissue Engineering. Part a·Joaquin CortiellaJoan E Nichols
Jun 26, 2010·Science·Thomas H PetersenLaura E Niklason
Jul 16, 2010·Nature Medicine·Harald C OttJoseph P Vacanti
Aug 31, 2010·The Journal of Biological Chemistry·Peter F BoveRichard C Boucher
Aug 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Micah R RogelKaren M Ridge
Sep 3, 2011·Tissue Engineering. Part a·Jessica A DeQuachKaren L Christman
Apr 10, 2012·Cell Stem Cell·Tyler A LongmireDarrell N Kotton
Sep 18, 2012·The International Journal of Biochemistry & Cell Biology·Roberto Espinosa Neira, Eduardo Perez Salazar
Oct 26, 2012·Methods in Molecular Biology·Robert F Gonzalez, Leland G Dobbs
Dec 4, 2012·American Journal of Respiratory Cell and Molecular Biology·Joo-Hyeon LeeCarla F Kim
Jan 9, 2013·Microvascular Research·Anna A BirukovaKonstantin G Birukov
Apr 22, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Yoko ItoRobert J Mason

❮ Previous
Next ❯

Citations

Aug 16, 2016·Current Opinion in Solid State & Materials Science·Gillie Agmon, Karen L Christman
Nov 1, 2016·Tissue Engineering. Part C, Methods·Andrew TrecartinTracy C Grikscheit
Jun 12, 2018·Organogenesis·Hideyori SengyokuTakeshi Nagayasu
Jan 13, 2018·Biotechnic & Histochemistry : Official Publication of the Biological Stain Commission·Z Vitenberga, M Pilmane
Jan 14, 2017·Journal of Tissue Engineering and Regenerative Medicine·Alexander J EnglerLaura E Niklason
Sep 27, 2015·American Journal of Physiology. Lung Cellular and Molecular Physiology·Collin Turner StablerPeter I Lelkes
Feb 14, 2017·Stem Cells Translational Medicine·Dan C WilkinsonBrigitte N Gomperts
Nov 12, 2015·Frontiers in Physiology·Sriram RavindranRaghuvaran Narayanan
Dec 14, 2016·Cell and Tissue Research·Collin T Stabler, Edward E Morrisey
Aug 29, 2019·F1000Research·Diane Lee, Mark Chambers

❮ Previous
Next ❯

Methods Mentioned

BETA
electron microscopy
fluorescence-activated cell sorting
bone marrow aspiration
PCR
FACS

Software Mentioned

iQ
Volocity
iCyler

Related Concepts

Related Feeds

Basement Membranes

Basement membranes are thin, specialized extracellular matrices surrounding most tissues in all metazoans. Here is the latest research on basement membranes.