Fate of Mycobacterium tuberculosis in peroxidase-loaded resting murine macrophages

International Journal of Mycobacteriology
Melby Dessiré Mendoza-AguilarOscar Rojas-Espinosa

Abstract

Myeloperoxidase (MPO), in the presence of hydrogen peroxide and a halide represent an efficient microbicidal mechanism of phagocytic cells. MPO is abundant in neutrophils which also respond to infection by producing large amounts of reactive oxygen species (ROS). MPO, ROS and halide constitute a very toxic antimicrobial system (called the Klebanoff system or KS). Resting mature macrophages do not contain granular MPO and thus are unable to kill pathogenic mycobacteria and some other microorganisms by this system. Under the hypothesis that transforming macrophages into peroxidase-positive (PO(+)) cells, these cells would be able to kill Mycobacterium tuberculosis, in this study, mature macrophages were loaded with exogenous peroxidase and were tested for their capacity to kill the Mycobacterium in the presence or in the absence of hydrogen peroxide. It was found that PO-loaded macrophages eagerly ingest M. tuberculosis, but do not show a significant mycobactericidal activity on this microorganism despite that it is highly susceptible to the Klebanoff system in vitro. Failure of PO-loaded macrophages to kill M. tuberculosis may obey either to an inappropriate location of the exogenous PO in these cells or more likely, to the pres...Continue Reading

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Citations

Dec 23, 2014·International Journal of Experimental Pathology·Mario Juarez-OrtegaOscar Rojas-Espinosa
Sep 21, 2017·Innate Immunity·Georgina Filio-RodríguezOscar Rojas-Espinosa
Mar 24, 2016·Journal of the American Association of Nurse Practitioners·Brenda C BuffingtonMichael R Zupan
May 22, 2019·Pharmaceuticals·Rafiou Agoro, Catherine Mura

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