Fate-restricted retinal progenitor cells adopt a molecular profile and spatial position distinct from multipotent progenitor cells

Developmental Biology
Diego F BuenaventuraMark M Emerson

Abstract

During development, multipotent retinal progenitor cells generate a large number of unique cell types. Recent evidence suggests that there are fate-restricted progenitor cell states in addition to multipotent ones. Here we report a transcriptomic analysis of fate- restricted progenitor cells biased to produce cone photoreceptors and horizontal cells, marked by the THRB cis-regulatory element ThrbCRM1. Comparison to a control population enriched in multipotent progenitor cells identified several genes considered to be pan-progenitor, such as VSX2, LHX2, and PAX6, as downregulated in these fate- restricted retinal progenitor cells. This differential regulation occurs in chick and in a different restricted progenitor population in mouse suggesting that this is a conserved feature of progenitor dynamics during retinal development. S-phase labeling also revealed that nuclear positions of restricted progenitor populations occupy distinct spatial niches within the developing chick retina. Using a conserved regulatory element proximal to the VSX2 gene, a potential negative feedback mechanism from specific transcription factors enriched in cone/horizontal cell progenitor cells was identified. This study identifies conserved molecular an...Continue Reading

Citations

Apr 12, 2019·Biology Open·Benjamin Souferi, Mark M Emerson
Jul 2, 2019·Investigative Ophthalmology & Visual Science·Diego F BuenaventuraMark M Emerson
Mar 21, 2020·Neural Development·Sruti PatooriMark M Emerson
Jul 7, 2019·International Journal of Molecular Sciences·Verónica Murcia-Belmonte, Lynda Erskine
Mar 29, 2021·Developmental Biology·Estie SchickMark M Emerson
Jun 22, 2021·IScience·Tahani W BaakdhahDerek van der Kooy

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