PMID: 6974505Sep 1, 1981Paper

Fatigue and metabolism of frog muscle fibers during stimulation and in response to caffeine

The American Journal of Physiology
V Nassar-GentinaS I Rapoport

Abstract

Tension and metabolite concentrations were measured in single frog muscle fibers at 15 degrees C in vitro, in response to electrical stimulation or to immersion in caffeine- or potassium chloride-Ringer. Sarcomere length equaled 2.3 micrometers. Interrupted stimulation for 150 s at 20 Hz or stimulation for 7.5 min at 1 Hz was followed by at least 20 min of fatigue, evidenced by a reduced 200-ms test contraction. Fatigued fibers contracted maximally in potassium chloride- or caffeine-Ringer. They had high lactate and glucose 6-phosphate concentrations and a reduced phosphocreatine (PCr) concentration. Adenosine 5'-triphosphate (ATP) concentration was approximately normal but was markedly reduced by a caffeine contracture. A plot of PCr consumption against the tension-time integral at different stimulation frequencies (25, 35, or 50 Hz) and durations had an intercept of 25.5 nmol PCr/mg protein at time zero and a corrected slope of 0.65 nmol approximately P/mg protein per kg . s . cm-2. Prolonged fatigue is not due to energy exhaustion or to the inability of muscle fibers to consume residual ATP but probably arises from long-lasting interference in excitation-contraction coupling, which can be reversed by KCl- or caffeine-induced...Continue Reading

References

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Citations

Oct 1, 1989·Clinical Physiology·H J Green, S R Jones
May 1, 1988·Acta Physiologica Scandinavica·J Lännergren, H Westerblad
Apr 25, 2000·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·D E Rassier, B R Macintosh
Oct 1, 1991·Journal of Muscle Research and Cell Motility·M C GarciaM Rozycka
Jan 1, 1987·European Journal of Applied Physiology and Occupational Physiology·J HirvonenM Härkönen
Jul 1, 1992·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·G O MathesonP S Allen
Oct 1, 1987·Muscle & Nerve·B R MacIntosh, C C Kupsh
Aug 13, 1999·Journal of Applied Physiology·T G Favero
Nov 9, 2018·Journal of Cell Science·Manuela LavoratoClara Franzini-Armstrong
Jan 1, 1991·Comparative Biochemistry and Physiology. A, Comparative Physiology·R A TurcotteP F Gardiner
Dec 1, 1991·Biochemical Medicine and Metabolic Biology·K A LuckinG A Klug

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