Fatty acid β-oxidation is required for the differentiation of larval hematopoietic progenitors in Drosophila.

ELife
Satish Kumar TiwariLolitika Mandal

Abstract

Cell-intrinsic and extrinsic signals regulate the state and fate of stem and progenitor cells. Recent advances in metabolomics illustrate that various metabolic pathways are also important in regulating stem cell fate. However, our understanding of the metabolic control of the state and fate of progenitor cells is in its infancy. Using Drosophila hematopoietic organ: lymph gland, we demonstrate that Fatty Acid Oxidation (FAO) is essential for the differentiation of blood cell progenitors. In the absence of FAO, the progenitors are unable to differentiate and exhibit altered histone acetylation. Interestingly, acetate supplementation rescues both histone acetylation and the differentiation defects. We further show that the CPT1/whd (withered), the rate-limiting enzyme of FAO, is transcriptionally regulated by Jun-Kinase (JNK), which has been previously implicated in progenitor differentiation. Our study thus reveals how the cellular signaling machinery integrates with the metabolic cue to facilitate the differentiation program.

References

Aug 1, 1997·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·M HollinsheadD J Vaux
Feb 25, 1998·Insect Biochemistry and Molecular Biology·H JiangM R Kanost
May 15, 2002·Current Biology : CB·Aurélie GrienenbergerJacques Pradel
Feb 6, 2003·Genes & Development·Tim LebestkyUtpal Banerjee
Jan 20, 2004·European Journal of Biochemistry·Kim Bartlett, Simon Eaton
Feb 19, 2004·Science's STKE : Signal Transduction Knowledge Environment·Sean E McGuireRonald L Davis
Apr 29, 2005·Development·Seung-Hye JungUtpal Banerjee
Sep 2, 2005·Molecular and Cellular Biology·Clément CarréChristophe Antoniewski
Sep 27, 2005·Journal of Biochemical and Biophysical Methods·Chenhui ZouZhufang Shen
Mar 17, 2007·Current Biology : CB·Eva KuruczIstván Andó
Sep 30, 2008·Biochemical and Biophysical Research Communications·Jing WuLi Fang
Mar 4, 2009·Cell Metabolism·Laura PalankerCarl S Thummel
May 23, 2009·Developmental Cell·Sergey A SinenkoUtpal Banerjee
May 23, 2009·Science·Kathryn E WellenCraig B Thompson
Aug 22, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·M SchreursF R van der Leij
Mar 3, 2010·Journal of Inherited Metabolic Disease·Sander Michel Houten, Ronald J A Wanders
Aug 17, 2010·Developmental Biology·Joanna KrzemienAlain Vincent
Apr 14, 2011·The Journal of Physiology·Kent Sahlin
May 13, 2011·Current Drug Metabolism·Jolanta PekalaTadeusz Librowski
May 25, 2011·World Journal of Stem Cells·Naiara Z SaraivaJoaquim M Garcia
Oct 11, 2011·Cell Stem Cell·Toshio SudaGregg L Semenza
Oct 12, 2011·Annual Review of Cell and Developmental Biology·Sophia Y Lunt, Matthew G Vander Heiden
Feb 22, 2012·Biochemical and Biophysical Research Communications·Yoshihito KishitaToshiro Aigaki
May 30, 2013·Development·Ng Shyh-ChangLewis C Cantley

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Citations

May 21, 2021·Frontiers in Cell and Developmental Biology·Satish Kumar Tiwari, Sudip Mandal
Aug 24, 2021·Frontiers in Cell and Developmental Biology·Sergey A SinenkoAlexey N Tomilin

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Methods Mentioned

BETA
acetylation
histone acetylation
dissection

Software Mentioned

Dome
Image J
Analyse Measure
Fiji
GraphPad
Imaris
ImageJ
EXCEL

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