Fatty acid alteration and the lateral diffusion of lipids in the plasma membrane of keratinocytes

Experimental Cell Research
R M FulbrightC L Marcelo

Abstract

The fluorescent probe diI was used to study the lateral mobility of lipids in in vitro strains of living adult human keratinocytes grown in four different media. One medium was essential fatty acid deficient (EFAD) and low in calcium ion, a medium known to yield cells that proliferate rapidly and contain lipid with extremely low levels of essential fatty acids. Two other media were supplemented with essential fatty acids (FAS), media that are known to result in cells that grow more slowly and have normalized fatty acid proportions. A fourth medium consisted of 1 microM all-trans-retinoic acid added to the fatty acid-supplemented medium (FAS-RA), a medium known to produce cells that are highly proliferative, with a growth rate greater than that of the FAS strains and similar to that of the EFAD strains. The keratinocytes grown in these four media were studied using the fluorescence recovery after photobleaching (FRAP) technique to determine the lateral diffusion rate of diI in the plasma membranes. Our results showed a positive correlation between growth rate and diffusion coefficient (D): the diffusion coefficient of diI was higher in the EFAD or FAS-RA cells than in the FAS cells. The measurement of D among the FAS cells fell ...Continue Reading

References

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Citations

Aug 21, 2013·Photosynthesis Research·Radek Kaňa
Feb 8, 2008·Molecular Biology of the Cell·Urszula GolebiewskaStuart McLaughlin
Oct 12, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hana ChoWon-Kyung Ho
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Jun 26, 2015·Scientific Reports·Daniel BlumenthalLevi A Gheber
Jun 25, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Theresa A HarrisonDonald B Hoover
Apr 17, 2014·Molecular Microbiology·Anja NenningerConrad W Mullineaux
Jul 18, 2003·Journal of Lipid Research·Georges DagherIsabelle Dugail
Oct 13, 2017·Scientific Reports·Masahito TanakaShigehiko Yumura
Mar 30, 2004·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Thomas WelssKlaus R Schröder

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