Fatty acid-binding protein3 expression in BeWo cells, a human placental choriocarcinoma cell line

Prostaglandins, Leukotrienes, and Essential Fatty Acids
Claire LeroyAsim K Duttaroy

Abstract

Cellular uptake of long chain fatty acids in human placental trophoblasts is thought to be mediated by several membrane- and cytoplasmic fatty acid-binding proteins (FABP). FABP3 was shown to be involved in long chain polyunsaturated fatty acids (LCPUFA) uptake in human trophoblastic choriocarcinoma cells, BeWo as the uptake of arachidonic acid,20:4n-6 (ARA) was decreased in FABP3-knockdown BeWo cells. However, the regulation of expression of FABP3 in these cells is not yet well known. The aim of the present study was to examine the FABP3 expression by LCPUFAs, insulin and LXR agonists in BeWo cells. Among all these fatty acids tested, only ARA dose-dependently stimulated the expression of FABP3 protein in these cells after 24h incubation while other fatty acids had no such effect. In addition, LXR agonist and insulin dose-dependently increased FABP3 protein expression in these cells after 24h incubation. Insulin-stimulated FABP3 protein expression was accompanied with an increased arachidonic acid uptake. Differentiated BeWo cells had lesser expression of FABP3 protein than in the undifferentiated cells as the cellular differentiation state was measured by hCG production. In preeclamptic placental tissue, lowered expression of...Continue Reading

Citations

Dec 21, 2018·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Véronique Ferchaud-RoucherTheresa L Powell
Feb 3, 2021·The FEBS Journal·Mingyu HuChao Tong

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