PMID: 9187305May 17, 1997Paper

Fatty acid binding proteins reduce 15-lipoxygenase-induced oxygenation of linoleic acid and arachidonic acid

Biochimica Et Biophysica Acta
B A EkA A Spector

Abstract

Free fatty acids in plasma and cells are mainly bound to membranes and proteins such as albumin and fatty acid binding proteins (FABP), which can regulate their biological activities and metabolic transformations. We have investigated the effect of FABP and albumin on the peroxidation of linoleic acid (18:2) and arachidonic acid (20:4) by 15-lipoxygenase (15-LO). Rabbit reticulocyte 15-LO produced a rapid conversion of [1-14C]18:2 to 13-hydroxyoctadecadienoic acid (13-HODE) and [3H]20:4 to 15-hydroxyeicosatetraenoic acid (15-HETE). 13-HODE formation was reduced when intestinal FABP (I-FABP). liver FABP (L-FABP) or albumin was added. The relative ability of these proteins to reduce 15-LO induced formation of 13-HODE and 15-HETE was BSA > L-FABP > I-FABP. Smaller reductions in activity were observed with 20:4 as compared to 18:2. The IC50-values of I-FABP and L-FABP, using either 18:2 (3.4 microM) or 20:4 (3.4 microM), were 4.6 +/- 0.6 and 1.9 +/- 0.2 microM, respectively, for reduction of 13-HODE and 6.8 +/- 0.3 and 3.1 +/- 0.2 microM, respectively, for reduction of 15-HETE formation. The smaller 15-HETE reduction correlated with decreased binding of 20:4 to the FABP. Titration calorimetry also showed that the I-FABP IC50 for 18...Continue Reading

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Citations

Jun 19, 1998·Chemistry and Physics of Lipids·F SchroederA Frolov
May 31, 2008·Nature Reviews. Drug Discovery·Masato Furuhashi, Gökhan S Hotamisligil
Apr 21, 2011·Human Genomics·Rebecca L Smathers, Dennis R Petersen
May 29, 2009·Journal of Lipid Research·Jing YanFrank J Burczynski
Apr 29, 2014·Biochimica Et Biophysica Acta·Laura RagonaHenriette Molinari
Sep 9, 2005·Current Opinion in Lipidology·Liza Makowski, Gökhan S Hotamisligil
Jul 11, 2006·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Frits Kamp, James A Hamilton
Oct 23, 2014·Experimental Lung Research·Hun Soo ChangChoon-Sik Park
Jun 12, 2010·The Journal of Nutritional Biochemistry·Barbara P AtshavesFriedhelm Schroeder
Aug 24, 2016·Biochemistry·Huan HuangFriedhelm Schroeder
Apr 20, 2017·The Journal of Endocrinology·Ricardo Rodríguez-CalvoLluís Masana
Sep 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Guqi WangFrank J Burczynski
Oct 8, 2015·Journal of Lipid Research·GuQi WangFrank J Burczynski
Dec 20, 2002·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Joern R SteinertRon Jacob
Apr 1, 2003·Cardiovascular Toxicology·Lex A MitchellRichard H Kennedy
Jun 5, 2007·Biochimica Et Biophysica Acta·Friedhelm SchroederAnn B Kier
Feb 6, 2008·Journal of Agricultural and Food Chemistry·Long Shuang HuangDai-Eun Sok

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