Fatty acid carbon isotope ratios in humans on controlled diets

Lipids
S K RheeJ T Brenna

Abstract

Carbon stable isotope ratios for six serum fatty acids (FA) are reported for human subjects on controlled fat diets to determine the range of natural isotope abundance and to demonstrate the leveling effect of a well-controlled diet. Twenty-nine subjects were randomly assigned to one of three controlled diets containing high, medium, or low fat. Diets were consumed for 8 wk. Serum samples were collected at baseline (0), 5, 6, 7, and 8 wk. FA were extracted and methylated. Isotope ratios were analyzed by high-precision gas chromatography combustion-isotope ratio mass spectrometry. At baseline, mean delta 13C for 16:0b, 16:1a, 18:0a, 18:1c,18:2n-6d and 20:4n-6bc were -24.1, -21.7, -21.6, -25.6, -29.6, and -25.0/1000, respectively, with an average standard deviation of 1.9/1000. Most delta 13C decreased during the diet period and appeared to have stabilized by week 5 at -25.3, -21.9, -22.3, -26.5, -30.1, and -24.5/1000, respectively. Between-subjected variability decreased from 1.74 to 1.20/1000 on the controlled diets. Measurement variability was 0.53/1000. The within-subject variability during weeks 5-8 was 0.57/1000 (range of 0.32-0.84/1000), showing a minimum biological fluctuation on controlled diets. There was no diet group ...Continue Reading

References

Jan 1, 1986·Biomedical & Environmental Mass Spectrometry·D MendelsohnG von La Chevallerie
Sep 1, 1988·Journal of Pediatric Gastroenterology and Nutrition·T W BouttonP D Klein
Feb 1, 1971·Biochemical Medicine·J N ThompsonT K Murray
Jun 1, 1983·Zeitschrift für Ernährungswissenschaft·S MetzlerG Wolfram
Sep 15, 1994·Analytical Chemistry·R J CaimiJ T Brenna
Aug 1, 1993·Analytical Chemistry·Z K GuoD Schoeller
Jul 1, 1995·Journal of Pediatric Gastroenterology and Nutrition·H DemmelmairB Koletzko
Feb 1, 1997·The American Journal of Clinical Nutrition·S K RheeJ T Brenna
Mar 26, 1998·Arteriosclerosis, Thrombosis, and Vascular Biology·H N GinsbergN Anderson

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Citations

Mar 11, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·W Meier-Augenstein
Feb 5, 2008·Steroids·Ulrich FlenkerWilhelm Schänzer
Oct 28, 2008·Rapid Communications in Mass Spectrometry : RCM·Rebecca A KraftChristopher D Saudek
Jul 12, 2017·Journal of Lipid Research·R J Scott LacombeRichard P Bazinet
Dec 6, 2018·The Journal of Nutrition·Gunter G C Kuhnle

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